State Key Lab of Supramolecular Structure and Materials, Jilin University, Changchun, 130012, PR China.
Nanoscale. 2011 May;3(5):2261-7. doi: 10.1039/c1nr10030k. Epub 2011 Apr 12.
Water-dispersed organic nanoparticles (NPs) constructed by the conjugated molecule 2,5,2',5'-tetra(4'-N,N-diphenylaminostyryl)biphenyl (DPA-TSB) with a high luminescence and large two-photon absorption (TPA) section were fabricated via the reprecipitation method. The average size of the NPs can be controlled from 40 nm to 80 nm by adjusting the reprecipitation conditions. The NPs in water dispersions showed high aggregative and optical stability, which were due to contributions from the special cruciform configuration and amorphous nature of DPA-TSB molecules. The cellular uptake behavior of DPA-TSB NPs was investigated to show their cell staining capabilities as nanoprobes using a confocal microscopy test in vitro. The results demonstrated that DPA-TSB NPs were readily internalized into cytoplasm with no apparent toxicity for up to 24 h, implying excellent imaging capabilities.
水相分散的有机纳米粒子（NPs）由具有高光致发光和大双光子吸收（TPA）截面的共轭分子 2,5,2',5'-四（4'-N,N-二苯基氨基苯乙烯基）联苯（DPA-TSB）通过再沉淀法制备。通过调节再沉淀条件，可以将 NPs 的平均尺寸从 40nm 控制到 80nm。在水中分散的 NPs 表现出高聚集和光学稳定性，这归因于 DPA-TSB 分子的特殊十字形构象和无定形性质。通过共聚焦显微镜测试体外研究了 DPA-TSB NPs 的细胞摄取行为，以显示它们作为纳米探针的细胞染色能力。结果表明，DPA-TSB NPs 很容易被内吞到细胞质中，在长达 24 小时内没有明显的毒性，这表明其具有优异的成像能力。
