Department of Immunology, Facultad de Medicina, UASLP, Ave. V. Carranza 2405, 78210, San Luis Potosí, SLP, Mexico.
J Clin Immunol. 2011 Aug;31(4):588-95. doi: 10.1007/s10875-011-9527-5. Epub 2011 Apr 13.
Rheumatoid arthritis (RA) is an autoimmune and inflammatory disease. Natural T regulatory (nTreg) cells, which constitutively express the CTLA-4 molecule, have an important role in the pathogenesis of autoimmune conditions. Although it has been reported that biological agents are able to modulate the levels or function of Treg lymphocytes, the possible effect of Abatacept (CTLA-4-Ig) therapy on these cells has not been studied in autoimmune conditions. We explored the effect of Abatacept therapy on Treg cells in patients with RA. The number of different subsets of Treg cells was analyzed by flow cytometry in the peripheral blood from 45 patients with RA that were (n = 30) or not (n = 15) under Abatacept therapy as well as in 20 healthy controls. The function of Treg cells was assessed by an assay of inhibition of lymphocyte proliferation. We found that Abatacept therapy was associated with a significant diminution in the levels of CD4+CD25(bright)Foxp3+, and CD4+CTLA-4+ nTreg cells. In contrast, the regulatory function of CD4+CD25+ lymphocytes was significantly enhanced after the administration of Abatacept. Our data suggest that CTLA-4-Ig exerts a complex and interesting effect on Treg cells in patients with RA.
类风湿关节炎（RA）是一种自身免疫性和炎症性疾病。天然调节性 T 细胞（nTreg）持续表达 CTLA-4 分子，在自身免疫性疾病的发病机制中具有重要作用。尽管已经报道生物制剂能够调节 Treg 淋巴细胞的水平或功能，但在自身免疫性疾病中尚未研究 Abatacept（CTLA-4-Ig）治疗对这些细胞的可能影响。我们探讨了 Abatacept 治疗对 RA 患者 Treg 细胞的影响。通过流式细胞术分析了来自 45 例 RA 患者（n = 30）或未接受 Abatacept 治疗（n = 15）以及 20 例健康对照者外周血中不同 Treg 细胞亚群的数量。通过抑制淋巴细胞增殖测定评估 Treg 细胞的功能。我们发现 Abatacept 治疗与 CD4+CD25（bright）Foxp3+和 CD4+CTLA-4+nTreg 细胞水平的显著降低相关。相比之下，Abatacept 给药后 CD4+CD25+淋巴细胞的调节功能显著增强。我们的数据表明 CTLA-4-Ig 对 RA 患者的 Treg 细胞产生复杂而有趣的影响。
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