Unitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Institut de Biomedicina (IBUB), Centros de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Universitat de Barcelona, Barcelona, Spain.
J Neurosci Res. 2011 Jul;89(7):1043-51. doi: 10.1002/jnr.22637. Epub 2011 Apr 12.
Taurine, 2-aminoethanesulfonic acid, is present at high concentrations in many invertebrate and vertebrate systems, and it has several biological functions. In addition, it has been related to a neuroprotective role against several diseases, such as epilepsy. It has been reported that taurine induces a decrease of calbindin-D28k, calretinin, and parvalbumin protein levels in the hippocampus 3 days after administration. In the present work we hypothesized that the decrease of these proteins could alter the action of kainic acid (KA) and make mice more susceptible to excitotoxicity. Therefore, we treated mice with taurine and after 3 days treated them with KA. The results showed that taurine pretreatment did not induce a major susceptibility to KA. Moreover, neurodegeneration was reduced in pretreated mice. However, astrogliosis was similar to that observed in mice treated only with KA. The immunohistochemistries for calbindin-D28k, calretinin, and parvalbumin showed that these proteins were reduced as a consequence of KA treatment and of taurine treatment. However, mice pretreated with taurine prior to KA administration presented the same reduction in these proteins as mice treated with only taurine or only KA.
牛磺酸，2-氨基乙磺酸，在许多无脊椎动物和脊椎动物系统中浓度很高，具有多种生物学功能。此外，它与几种疾病的神经保护作用有关，如癫痫。据报道，牛磺酸在给药后 3 天诱导海马中钙结合蛋白-D28k、钙调蛋白和钙结合蛋白的蛋白水平降低。在本工作中，我们假设这些蛋白质的减少会改变海人酸 (KA) 的作用，使小鼠更容易受到兴奋性毒性的影响。因此，我们用牛磺酸处理小鼠，3 天后用 KA 处理它们。结果表明，牛磺酸预处理不会引起对 KA 的易感性增加。此外，预处理小鼠的神经退行性变减少。然而，星形胶质细胞增生与仅用 KA 处理的小鼠观察到的相似。钙结合蛋白-D28k、钙调蛋白和钙结合蛋白的免疫组织化学显示，这些蛋白质因 KA 处理和牛磺酸处理而减少。然而，在给予 KA 之前用牛磺酸预处理的小鼠在这些蛋白质中的减少与仅用牛磺酸或仅用 KA 处理的小鼠相同。
