Widney Daniel P, Gui Dorina, Popoviciu Laura M, Said Jonathan W, Breen Elizabeth C, Huang Xin, Kitchen Christina M R, Alcantar Juan M, Smith Jeffrey B, Detels Roger, Martínez-Maza Otoniel
Department of Obstetrics & Gynecology, David Geffen School of Medicine at UCLA, CA 90095-1740, USA.
AIDS Res Treat. 2010;2010:164586. doi: 10.1155/2010/164586. Epub 2010 Aug 25.
Background. The homeostatic chemokine, CXCL13 (BLC, BCA-1), helps direct the recirculation of mature, resting B cells, which express its receptor, CXCR5. CXCL13/CXCR5 are expressed, and may play a role, in some non-AIDS-associated B cell tumors. Objective. To determine if CXCL13/CXCR5 are associated with AIDS-related non-Hodgkin's lymphoma (AIDS-NHL). Methods. Serum CXCL13 levels were measured by ELISA in 46 subjects who developed AIDS-NHL in the Multicenter AIDS Cohort Study and in controls. The expression or function of CXCL13 and CXCR5 was examined on primary AIDS-NHL specimens or AIDS-NHL cell lines. Results. Serum CXCL13 levels were significantly elevated in the AIDS-NHL group compared to controls. All primary AIDS-NHL specimens showed CXCR5 expression and most also showed CXCL13 expression. AIDS-NHL cell lines expressed CXCR5 and showed chemotaxis towards CXCL13. Conclusions. CXCL13/CXCR5 are expressed in AIDS-NHL and could potentially be involved in its biology. CXCL13 may have potential as a biomarker for AIDS-NHL.
背景。稳态趋化因子CXCL13（BLC，BCA-1）有助于指导表达其受体CXCR5的成熟静止B细胞的再循环。CXCL13/CXCR5在一些非艾滋病相关B细胞肿瘤中表达，且可能发挥作用。目的。确定CXCL13/CXCR5是否与艾滋病相关非霍奇金淋巴瘤（AIDS-NHL）有关。方法。在多中心艾滋病队列研究中，采用酶联免疫吸附测定法（ELISA）检测46例发生AIDS-NHL的受试者及对照者血清CXCL13水平。对原发性AIDS-NHL标本或AIDS-NHL细胞系检测CXCL13和CXCR5的表达或功能。结果。与对照相比，AIDS-NHL组血清CXCL13水平显著升高。所有原发性AIDS-NHL标本均显示CXCR5表达，且大多数也显示CXCL13表达。AIDS-NHL细胞系表达CXCR5，并对CXCL13表现出趋化性。结论。CXCL13/CXCR5在AIDS-NHL中表达，可能参与其生物学过程。CXCL13可能有潜力作为AIDS-NHL的生物标志物。
