Bambou Jean-Christophe, Gourdine Jean-Luc, Grondin Roxanne, Vachiery Nathalie, Renaudeau David
INRA UR143, Unité de Recherches Zootechniques, Centre INRA Antilles Guyane, Domaine de Duclos, Prise d'Eau, 97170 Petit Bourg, Guadeloupe, French West Indies, France.
Trop Anim Health Prod. 2011 Dec;43(8):1535-41. doi: 10.1007/s11250-011-9838-9. Epub 2011 Apr 14.
We evaluated the effect of heat challenge on cell viability, concanavalin A-induced proliferation and heat shock protein (HSPs) mRNA expression in peripheral mononuclear blood cells (PBMC) isolated from Creole (CR) and Large White (LW) pigs. The PBMCs were cultured for 9 h at 37 °C before being subjected to heat challenge: (1) at 42 °C or 45 °C for 2, 4, 6 and 9 h to monitor cell viability;(2) at 45 °C for 2 and 9 h followed by stimulation for 24 h at 37 °C with concanavalin A to evaluate mitogen-induced proliferation; and (3) at 45 °C for 3, 6 and 9 h to measure induction of HSP70.2 and HSP90 mRNA. Cell viability was affected by breed and temperature (P < 0.01), and the viability decrease caused by heat challenge was greater for LW than CR pigs. For mitogen-stimulated PBMCs, incubation at 45 °C reduced lymphoblastogenesis equally in both breeds (P < 0.01). Although heat challenge for 3 and 6 h at 45°C induced expression of HSP70.2 and HSP90 mRNA, no breed difference was observed. In conclusion, differences in heat resistance between these two breeds at the whole organism level are reflected at the cellular level. Neither HSP70.2 nor HSP90 mRNA expression levels explain this effect.
我们评估了热应激对从克里奥尔猪（CR）和大白猪（LW）分离的外周血单个核细胞（PBMC）的细胞活力、伴刀豆球蛋白A诱导的增殖以及热休克蛋白（HSPs）mRNA表达的影响。PBMC在37°C培养9小时后进行热应激处理：（1）在42°C或45°C分别处理2、4、6和9小时以监测细胞活力；（2）在45°C处理2和9小时，随后在37°C用伴刀豆球蛋白A刺激24小时以评估有丝分裂原诱导的增殖；（3）在45°C处理3、6和9小时以测量HSP70.2和HSP90 mRNA的诱导情况。细胞活力受品种和温度影响（P<0.01），热应激导致的活力下降在LW猪中比CR猪更大。对于有丝分裂原刺激的PBMC，在45°C孵育对两个品种的淋巴细胞生成抑制作用相同（P<0.01）。虽然在45°C热应激3和6小时诱导了HSP70.2和HSP90 mRNA的表达，但未观察到品种差异。总之，这两个品种在整体水平上的耐热性差异在细胞水平上得到体现。HSP70.2和HSP90 mRNA的表达水平均不能解释这种效应。
