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钙参与了一氧化氮和生长素诱导的水稻侧根形成。

Calcium is involved in nitric oxide- and auxin-induced lateral root formation in rice.

机构信息

Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.

出版信息

Protoplasma. 2012 Jan;249(1):187-95. doi: 10.1007/s00709-011-0277-2. Epub 2011 Apr 14.

Abstract

In the present study, the role of nitric oxide (NO) in the regulation of lateral root (LR) formation in rice was examined. Application of sodium nitroprusside (SNP; a NO donor) and indole-3-butyric acid (IBA; a naturally occurring auxin) to rice seedlings induced LR formation. The effect is specific for NO because the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3- oxide (cPTIO) blocked the action of SNP and IBA. Endogenous NO was detected by the specific fluorescence probe 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate. SNP- and IBA-induced NO fluorescence was specifically suppressed by cPTIO. Nitrate reductase (NR) inhibitor sodium tungstate completely inhibited IBA-induced LR formation and NO fluorescence. However, nitric oxide synthase inhibitor N (G)-nitro-L: -arginine methyl ester hydrochloride slightly reduced IBA-induced LR formation and NO generation. It appears that NO generation that occurs in response to IBA might primarily involve NR activity. Moreover, NO production caused by SNP and IBA was localized in root area corresponding to LR emergence. The effects of Ca(2+) chelators, Ca(2+)-channel inhibitors, and calmodulin antagonists on LR formation induced by SNP and IBA were also examined. All these inhibitors were effective in reducing the action of SNP and IBA. However, Ca(2+) chelators and Ca(2+)-channel inhibitors had no effect on SNP- and IBA-induced NO generation. It is concluded that cytosolic levels of Ca(2+) may regulate SNP and IBA action through calmodulin-dependent mechanism.

摘要

在本研究中,考察了一氧化氮(NO)在调控水稻侧根(LR)形成中的作用。向水稻幼苗施用硝普钠(SNP;NO 供体)和吲哚-3-丁酸(IBA;一种天然存在的生长素)诱导了 LR 的形成。这种作用是特异的,因为 NO 清除剂 2-(4-羧基苯基)-4,4,5,5-四甲基恶唑啉-1-氧-3-氧化物(cPTIO)阻断了 SNP 和 IBA 的作用。通过特异性荧光探针 4-氨基-5-甲基氨基-2',7'-二氟荧光素二乙酸酯检测内源性 NO。SNP 和 IBA 诱导的 NO 荧光被 cPTIO 特异性抑制。硝酸还原酶(NR)抑制剂钨酸钠完全抑制 IBA 诱导的 LR 形成和 NO 荧光。然而,一氧化氮合酶抑制剂 N(G)-硝基-L:-精氨酸甲酯盐酸盐略微降低了 IBA 诱导的 LR 形成和 NO 的产生。似乎 NR 活性主要涉及 IBA 诱导的 NO 生成。此外,SNP 和 IBA 引起的 NO 产生定位于与 LR 出现相对应的根区。还研究了 Ca(2+)螯合剂、Ca(2+)通道抑制剂和钙调蛋白拮抗剂对 SNP 和 IBA 诱导的 LR 形成的影响。所有这些抑制剂都有效地降低了 SNP 和 IBA 的作用。然而,Ca(2+)螯合剂和 Ca(2+)通道抑制剂对 SNP 和 IBA 诱导的 NO 产生没有影响。结论是细胞溶质 Ca(2+)水平可能通过钙调蛋白依赖性机制调节 SNP 和 IBA 的作用。

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