人类胸苷激酶2活性的数学模型。
A mathematical model of human thymidine kinase 2 activity.
作者信息
Radivoyevitch T, Munch-Petersen B, Wang L, Eriksson S
机构信息
Department of Epidemiology and Biostatistics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2011 Mar;30(3):203-9. doi: 10.1080/15257770.2011.563765.
Abstract
The mitochondrial enzyme thymidine kinase 2 (TK2) phosphorylates deoxythymidine (dT) and deoxycytidine (dC) to form dTMP and dCMP, which in cells rapidly become the negative-feedback end-products dTTP and dCTP. TK2 kinetic activity exhibits Hill coefficients of ∼0.5 (apparent negative cooperativity) for dT and ∼1 for dC. We present a mathematical model of TK2 activity that is applicable if TK2 exists as two monomer forms in equilibrium.
摘要
线粒体酶胸苷激酶2（TK2）将脱氧胸苷（dT）和脱氧胞苷（dC）磷酸化，形成dTMP和dCMP，而在细胞中它们会迅速转变为负反馈终产物dTTP和dCTP。TK2的动力学活性对dT表现出约0.5的希尔系数（表观负协同性），对dC表现出约1的希尔系数。我们提出了一个TK2活性的数学模型，该模型适用于TK2以两种单体形式处于平衡状态的情况。
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