Department of Genetics, Biology and Biochemistry, University of Turin, Turin, Italy.
Cell Biochem Funct. 2011 Jun;29(4):311-33. doi: 10.1002/cbf.1751.
The particular combination of polydeoxyribonucleotides, l-carnitine, calcium ions, proteolytic enzyme and other ingredients acts in a synergetic way in the regeneration of skin and connective tissues. This new formulation of active principles was tested in vitro as a cell and tissue culture medium and in vivo for various preparations in support of tissue regeneration. In vitro, the new blend allowed the maintenance of skin biopsies for more than 1 year in eutrophic conditions. Immunocytochemical analyses of fibroblasts isolated from these biopsies confirmed a significant increase of the epidermal and connective wound-healing markers such as collagen type I, collagen type IV, cytokeratin 1 (CK1), CK5, CK10 and CK14 versus controls. To examine the effects of the new compound in vivo, we studied impaired wound healing in genetically diabetic db/db mice. At day 18, diabetic mice treated with the new composition showed 100% closure of wounds and faster healing than mice treated with the other solutions. This complex of vital continuity factors or life-keeping factors could be used as a tissue-preserving solution or a cosmetic/drug/medical device to accelerate wound healing in the treatment of patients with deficient wound repair to promote the regeneration of cutaneous and connective tissues (injuries-wound, dermatitis) and prevent the recurrent relapses.
聚脱氧核糖核苷酸、左旋肉碱、钙离子、蛋白酶和其他成分的特定组合在皮肤和结缔组织的再生中协同作用。这种新的活性成分配方在体外作为细胞和组织培养基进行了测试，并在体内用于各种制剂以支持组织再生。在体外，新的混合物使肥胖条件下的皮肤活检维持超过 1 年。从这些活检中分离出的成纤维细胞的免疫细胞化学分析证实，表皮和结缔组织愈合标志物（如胶原蛋白 I、IV 型、细胞角蛋白 1（CK1）、CK5、CK10 和 CK14）的显著增加与对照相比。为了研究新化合物在体内的作用，我们研究了遗传糖尿病 db/db 小鼠的创伤愈合受损。在第 18 天，用新组合物治疗的糖尿病小鼠的伤口闭合率达到 100%，比用其他溶液治疗的小鼠愈合更快。这种生命连续性因素或维持生命因素的复合物可作为组织保存溶液或美容/药物/医疗器械，用于加速伤口愈合，治疗伤口修复不足的患者，促进皮肤和结缔组织（伤口、皮炎）的再生，并预防复发性复发。
