University of Manchester, Manchester Academic Health Science Centre, NIHR Translational Research Facility in Respiratory Medicine, University Hospital of South Manchester NHS Foundation Trust, Manchester, United Kingdom.
J Allergy Clin Immunol. 2011 Jun;127(6):1480-5. doi: 10.1016/j.jaci.2011.03.014. Epub 2011 Apr 13.
An increase in IgE antibody levels to inhalant allergens is associated with an increased likelihood of wheezing. The role of allergen-specific IgG and IgG4 in relation to wheezing is yet to be determined.
We sought to investigate whether Fel d 1-specific IgG and IgG4 antibodies modify the association between cat allergen-specific IgE and childhood wheezing.
We used data from 2 population-based birth cohorts (United Kingdom [UK], n = 473; Australia, n = 1336). Current wheeze was defined as wheezing in the previous 12 months at age 5 (UK) and 14 (Australia) years. We determined cat allergen-specific IgE (whole extract) and IgG and IgG4 antibody (purified rFel d 1) levels and used logistic regression to estimate the relationship between wheeze and the quantitative allergen antibody levels.
In the univariate analysis risk of wheezing increased significantly with increasing cat-specific IgE levels (UK: odds ratio [OR], 1.56; 95% CI, 1.28-1.90; Australia: OR, 1.29; 95% CI, 1.19-1.40). rFel d 1-specific IgG or IgG4 had no significant effect on wheeze in either population. However, a different pattern of the relationship between antibody levels and wheezing emerged in the multivariate analysis. In the UK cat-specific IgE increased the risk of wheeze (OR, 2.01; 95% CI, 1.29-3.12; P = .002), whereas rFel d 1-specific IgG decreased the risk (OR, 0.46; 95% CI, 0.21-0.99; P = .05). This finding was replicated in Australia (IgE: OR, 1.46; 95% CI, 1.28-1.68; P < .001; IgG: OR, 0.66; 95% CI, 0.44-0.99; P = .049). There was no significant association between IgG4 antibody levels and wheezing in either population.
rFel d 1-specific IgG but not IgG4 antibody levels significantly modify the association between cat-specific IgE and childhood wheezing, with the risk of symptoms decreasing with increasing IgG levels.
免疫球蛋白 E（IgE）抗体水平对吸入性过敏原的增加与喘息的可能性增加有关。过敏原特异性 IgG 和 IgG4 与喘息的关系尚待确定。
我们旨在研究猫过敏原特异性 IgG 和 IgG4 抗体是否会改变猫过敏原特异性 IgE 与儿童喘息之间的关联。
我们使用了来自两个基于人群的出生队列（英国[UK]，n=473；澳大利亚，n=1336）的数据。当前喘息定义为 5 岁（英国）和 14 岁（澳大利亚）时过去 12 个月内的喘息。我们测定了猫过敏原特异性 IgE（全提取物）和 IgG 和 IgG4 抗体（纯化的 rFel d 1）水平，并使用逻辑回归估计了喘息与定量过敏原抗体水平之间的关系。
在单变量分析中，喘息的风险随着猫特异性 IgE 水平的升高而显著增加（英国：优势比[OR]，1.56；95%置信区间[CI]，1.28-1.90；澳大利亚：OR，1.29；95%CI，1.19-1.40）。rFel d 1 特异性 IgG 或 IgG4 对两个人群的喘息均无显著影响。然而，在多变量分析中，抗体水平与喘息之间的关系呈现出不同的模式。在英国，猫特异性 IgE 增加了喘息的风险（OR，2.01；95%CI，1.29-3.12；P=.002），而 rFel d 1 特异性 IgG 降低了风险（OR，0.46；95%CI，0.21-0.99；P=.05）。这一发现在澳大利亚得到了复制（IgE：OR，1.46；95%CI，1.28-1.68；P<.001；IgG：OR，0.66；95%CI，0.44-0.99；P=.049）。在两个人群中，IgG4 抗体水平与喘息均无显著关联。
rFel d 1 特异性 IgG 但不是 IgG4 抗体水平显著改变了猫特异性 IgE 与儿童喘息之间的关联，随着 IgG 水平的升高，症状的风险降低。
