用于单细胞操作的隔膜皮升泵。
Diaphragm pico-liter pump for single-cell manipulation.
机构信息
Mechanical Design and Production Engineering, Cairo University, Giza, 12316, Egypt.
出版信息
Biomed Microdevices. 2011 Aug;13(4):651-9. doi: 10.1007/s10544-011-9535-5.
A pico-liter pump is developed and integrated into a robotic manipulation system that automatically selects and transfers individual living cells of interest to analysis locations. The pump is a displacement type pump comprising one cylindrical chamber connected to a capillary micropipette. The top of the chamber is a thin diaphragm which, when deflected, causes the volume of the fluid-filled cylindrical chamber to change thereby causing fluid in the chamber to flow in and out of the micropipette. This enables aspirating and dispensing individual living cells. The diaphragm is deflected by a piezoelectric actuator that pushes against its center. The pump aspirates and dispenses volumes of fluid between 500 pL and 250 nL at flow rates up to 250 nL/s. The piezo-driven diaphragm arrangement provides exquisite control of the flow rate in and out of the capillary orifice. This feature, in turn, allows reduced perturbation of live cells by controlling and minimizing the applied shear stresses.
研制了一种皮升泵，并将其集成到一个机器人操作系统中，该系统可自动选择和将有兴趣的单个活细胞转移到分析位置。该泵是一种位移式泵，由一个与毛细管微吸管相连的圆柱形腔室组成。腔室的顶部是一个薄的膜片，当膜片发生偏转时，会导致充满流体的圆柱形腔室的体积发生变化，从而使腔室内的流体进出微吸管。这使得能够抽吸和分配单个活细胞。膜片由一个压电器件驱动，压电器件压在膜片的中心使其发生偏转。该泵可在 500 pL 至 250 nL 之间的体积范围内以高达 250 nL/s 的流速抽吸和分配流体。压电器件驱动的膜片装置可对毛细管孔内外的流速进行精密控制。这一特性反过来又通过控制和最小化施加的剪切应力来减少对活细胞的干扰。
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