现场报道：基因编码荧光报告蛋白揭示活体生物系统中的信号动态。
Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.
机构信息
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
出版信息
Annu Rev Biochem. 2011;80:375-401. doi: 10.1146/annurev-biochem-060409-093259.
Abstract
Real-time visualization of a wide range of biochemical processes in living systems is being made possible through the development and application of genetically encoded fluorescent reporters. These versatile biosensors have proven themselves tailor-made to the study of signal transduction, and in this review, we discuss some of the unique insights that they continue to provide regarding the spatial organization and dynamic regulation of intracellular signaling networks. In addition, we explore the more recent push to expand the scope of biological phenomena that can be monitored using these reporters, while also considering the potential to integrate this highly adaptable technology with a number of emerging techniques that may significantly broaden our view of how networks of biochemical processes shape larger biological phenomena.
摘要
通过开发和应用基因编码荧光报告蛋白，实时可视化活细胞内各种生化过程成为可能。这些多功能生物传感器已被证明非常适合用于研究信号转导，在这篇综述中，我们将讨论它们在细胞内信号网络的空间组织和动态调控方面提供的一些独特见解。此外，我们还探讨了最近利用这些报告蛋白来扩展可监测的生物现象范围的趋势，同时考虑将这项高度适应性强的技术与一些新兴技术相结合的可能性，这可能会极大地拓宽我们对生化过程网络如何塑造更大的生物学现象的认识。
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