Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Fish Shellfish Immunol. 2011 Jun;30(6):1249-56. doi: 10.1016/j.fsi.2011.03.024. Epub 2011 Apr 9.
Both cDNA library mining and transcriptome analysis were used to obtain 21 immunoglobulin light chain (IgL) sequences for the large yellow croaker, Pseudosciaena crocea. Full-length cDNA sequences are available for 10 of these, and they were identified as belonging to the three IgL isotypes of LycIgL1, LycIgL2, and LycIgL3. The LycIgL1 isotype is most abundant in the large yellow croaker IgL repertoire, as in the other teleosts. Tissue expression profile analysis revealed that the three LycIgL isotypes were constitutively expressed at different abundances in the kidney, spleen, liver, gill, heart, intestine, and muscle, although the heart did not express LycIgL3. Real-time polymerase chain reaction revealed that expression of the three LycIgL isotypes in the kidney and spleen tissues was up-regulated during 72 h of inductions with poly(I:C) or bacterial vaccine at different intensities and in different manners. The LycIgL1 isotype responded to stimulations most intensely in the spleen, while the LycIgL3 isotype responded most quickly in the kidney. Compared to the LycIgL1 and LycIgL3 isotypes, the LycIgL2 isotype responded more slowly and weakly in both tissues. These results indicate different isotypes of LycIgL respond to immune stimuli in the spleen and kidney in an isotypic-specific manner.
通过 cDNA 文库挖掘和转录组分析，获得了大黄鱼（Pseudosciaena crocea）的 21 条免疫球蛋白轻链（IgL）序列。其中 10 条全长 cDNA 序列已被鉴定为属于 LycIgL1、LycIgL2 和 LycIgL3 三种 IgL 同种型。在大黄鱼 IgL 库中，LycIgL1 同种型最为丰富，这与其他硬骨鱼类相同。组织表达谱分析显示，三种 LycIgL 同种型在肾脏、脾脏、肝脏、鳃、心脏、肠和肌肉中以不同的丰度持续表达，尽管心脏不表达 LycIgL3。实时聚合酶链反应显示，在肾脏和脾脏组织中，三种 LycIgL 同种型在不同强度和方式的 poly(I:C)或细菌疫苗诱导 72 小时内的表达均上调。LycIgL1 同种型在脾脏中的反应最为强烈，而 LycIgL3 同种型在肾脏中的反应最快。与 LycIgL1 和 LycIgL3 同种型相比，LycIgL2 同种型在两种组织中的反应速度较慢且较弱。这些结果表明，不同的 LycIgL 同种型以同种型特异性的方式在脾脏和肾脏中对免疫刺激做出反应。
