College of Life Sciences, Tianjin Normal University, Tianjin 300387, PR China.
Fish Shellfish Immunol. 2011 Jun;30(6):1362-7. doi: 10.1016/j.fsi.2011.03.023. Epub 2011 Apr 7.
Interleukin-8 (IL-8), or CXCL8, is a member of the CXC chemokine family that in mammals is known to mediate inflammatory response. In this study, we identified and analyzed an IL-8 orthologue, CsIL8, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsIL8 contains 100 residues and is closely related to the lineage 1 IL-8 of a number of fish species. In silico analysis identified in CsIL8 a CXC chemokine domain that contains four conserved cysteine residues, two of which form the CXC signature motif of CXC chemokines. Expression of CsIL8 as determined by quantitative real time RT-PCR was detected in a wide range of tissues under normal physiological conditions and was upregulated by bacterial challenge and by vaccination with a subunit vaccine. Purified recombinant CsIL8 (rCsIL8) induced migration of peripheral blood leukocytes and head kidney (HK) lymphocytes and stimulated the proliferation of these cells in a dose-dependent manner. When rCsIL8 was added to the cell culture of HK lymphocytes, it upregulated the expression of interleukin-1β and CsIL8 in a time-dependent fashion. These results indicate that CsIL8 is a biologically active CXC chemokine with immunoregulatory activity and that CsIL8 is involved in pathogen-induced inflammatory response.
白细胞介素-8（IL-8），也称为 CXCL8，是哺乳动物中 CXC 趋化因子家族的一员，已知其介导炎症反应。在本研究中，我们从半滑舌鳎（Cynoglossus semilaevis）中鉴定并分析了一个 IL-8 同源物，CsIL8。CsIL8 的推导氨基酸序列包含 100 个残基，与许多鱼类物种的 1 型 IL-8 密切相关。计算机分析在 CsIL8 中鉴定出一个 CXC 趋化因子结构域，该结构域包含四个保守的半胱氨酸残基，其中两个形成 CXC 趋化因子的 CXC 特征基序。定量实时 RT-PCR 检测到 CsIL8 在正常生理条件下在广泛的组织中表达，并在细菌挑战和亚单位疫苗接种后上调。纯化的重组 CsIL8（rCsIL8）诱导外周血白细胞和头肾（HK）淋巴细胞迁移，并以剂量依赖性方式刺激这些细胞的增殖。当 rCsIL8 添加到头肾淋巴细胞的细胞培养物中时，它以时间依赖性方式上调白细胞介素-1β和 CsIL8 的表达。这些结果表明 CsIL8 是一种具有免疫调节活性的生物活性 CXC 趋化因子，并且 CsIL8 参与了病原体诱导的炎症反应。
