Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Theriogenology. 2011 Aug;76(3):409-18. doi: 10.1016/j.theriogenology.2011.02.016. Epub 2011 Apr 14.
Three ejaculates from each of eight stallions were subjected to cryopreservation in a milk/egg yolk-based freezing extender or an egg yolk-based freezing extender. Semen was exposed to a fast prefreeze cooling rate (FAST; semen immediately subjected to cryopreservation) or a slow prefreeze cooling rate (SLOW; semen pre-cooled at a controlled rate for 80 min prior to cryopreservation). Postthaw semen was diluted in initial freezing medium (FM) or INRA 96 (IMV Technologies, L'Aigle, France) prior to analysis of 10 experimental end points: total motility (MOT; %), progressive motility (PMOT; %), curvilinear velocity (VCL; μm/s), linearity (LIN; %), intact acrosomal and plasma membranes (AIMI; %), intact acrosomal membranes (AI; %), intact plasma membranes (MI; %), and DNA quality. Eight of 10 experimental endpoints (MOT, PMOT, average-path velocity [VAP], mean straight-line velocity [VSL], LIN AIMI, AI, and MI) were affected by extender type, with egg yolk-based extender yielding higher values than milk/egg yolk-based extender (P < 0.05). Exposure of extended semen to a slow prefreeze cooling period resulted in increased values for six of eight endpoints (MOT, PMOT, VCL, AIMI, AI, and MI), as compared with a fast prefreeze cooling period (P < 0.05). As a postthaw diluent, INRA 96 yielded higher mean values than FM for MOT, PMOT, VCL, average-path velocity, and mean straight-line velocity (P < 0.05). Treatment group FM yielded slightly higher values than INRA 96 for LIN and MI (P < 0.05). In conclusion, a slow prefreeze cooling rate was superior to a fast prefreeze cooling rate, regardless of freezing extender used, and INRA 96 served as a satisfactory postthaw diluent prior to semen analysis.
将八头种马的每头种马的三个射精精液分别置于基于奶/卵黄的冷冻液或卵黄基冷冻液中进行冷冻保存。精液暴露于快速预冷冻冷却速率（FAST；精液立即进行冷冻保存）或缓慢预冷冻冷却速率（SLOW；精液在冷冻保存前以受控速率预冷却 80 分钟）。解冻后，精液在初始冷冻液（FM）或 INRA 96（IMV Technologies，L'Aigle，法国）中稀释，然后分析 10 个实验终点：总活力（MOT；%）、前向运动活力（PMOT；%）、曲线速度（VCL；μm/s）、直线性（LIN；%）、完整顶体和质膜（AIMI；%）、完整顶体膜（AI；%）、完整质膜（MI；%）和 DNA 质量。10 个实验终点中的 8 个（MOT、PMOT、平均路径速度[VAP]、平均直线速度[VSL]、LIN AIMI、AI 和 MI）受到扩展器类型的影响，卵黄基扩展器产生的数值高于奶/卵黄基扩展器（P<0.05）。与快速预冷冻冷却期相比，延长精液暴露于缓慢预冷冻冷却期导致八个终点中的六个（MOT、PMOT、VCL、AIMI、AI 和 MI）的值增加（P<0.05）。与 FM 相比，INRA 96 作为解冻后的稀释液，MOT、PMOT、VCL、平均路径速度和平均直线速度的平均值更高（P<0.05）。处理组 FM 对于 LIN 和 MI 的值略高于 INRA 96（P<0.05）。总之，无论使用哪种冷冻液，缓慢的预冷冻冷却速率均优于快速预冷冻冷却速率，并且 INRA 96 在精液分析之前是一种令人满意的解冻后稀释液。
