Institute of Chemical Technology, Department of Analytical Chemistry, Prague, Czech Republic.
J Chromatogr A. 2011 May 20;1218(20):3029-36. doi: 10.1016/j.chroma.2011.03.057. Epub 2011 Apr 2.
A new type of high performance liquid chromatography (HPLC) stationary phase was prepared, and its chromatographic properties were evaluated. The sorbent was composed of metallacarborane covalently bound to silica. Because of the chemical structure of the immobilized metallacarborane, the synthesized stationary phase was able to interact with nonpolar analytes via hydrophobic interactions. The chromatographic behavior of several low-molecular-weight hydrocarbons on the sorbent under typical reversed-phase conditions was compared with octadecyl-, sulfo phenyl- and aminopropyl-modified silica stationary phases. Moreover, as a consequence of the synthetic protocol employed, the immobilization of the metallacarborane led to the development of a zwitterionic chemically bonded phase, which demonstrated excellent resistance to "phase collapse" in a 100% aqueous environment. Finally, preliminary experiments indicated that the new stationary phase has the potential for utilization in hydrophilic interaction chromatography (HILIC) mode for the separation of polar compounds.
一种新型高效液相色谱（HPLC）固定相被制备，并对其色谱性能进行了评价。该固定相由金属碳硼烷键合到硅胶上组成。由于固定化金属碳硼烷的化学结构，合成的固定相能够通过疏水相互作用与非极性分析物相互作用。在典型的反相条件下，几种低分子量烃在固定相上的色谱行为与十八烷基、磺基苯基和氨丙基改性硅胶固定相进行了比较。此外，由于所采用的合成方案，金属碳硼烷的固定化导致了两性离子键合相的发展，该相在 100%水环境中表现出优异的抗“相塌陷”能力。最后，初步实验表明，新型固定相有可能用于亲水相互作用色谱（HILIC）模式下分离极性化合物。
