Department of Clinical Investigation, Tripler Army Medical Center, Honolulu, HI, USA.
Am J Obstet Gynecol. 2011 Jul;205(1):82.e1-9. doi: 10.1016/j.ajog.2011.02.045. Epub 2011 Feb 23.
The objective of the study was to compare the influence of collagen-coated vs uncoated polypropylene meshes on the expression of genes critical for wound healing.
In 54 rats, abdominal wall defects were created, repaired by polypropylene sutures, and covered by an overlay of coated polypropylene (n = 20), uncoated polypropylene (n = 18), or no mesh (n = 16). Explants were harvested 7 or 90 days after repair and divided for histological, immunohistochemical, and messenger ribonucleic acid (mRNA) analyses. Real-time quantitative polymerase chain reaction arrays were used to profile the expression of 84 genes at the tissue-mesh interface.
One week after implantation, coated mesh elicited a slightly greater inflammatory response and increased mRNA expression of 4 proinflammatory cytokines compared with uncoated mesh. Both materials, however, induced a comparable expression of cytokines and matrix metalloproteinases relative to suture repair 90 days after implantation.
Collagen-coated polypropylene mesh induces elevated inflammatory cytokine expression compared with uncoated mesh early in the healing process, but the response to both meshes is similar 90 days after implantation.
本研究旨在比较胶原蛋白涂层和未涂层的聚丙烯网片对伤口愈合关键基因表达的影响。
在 54 只大鼠中，建立腹壁缺损模型，用聚丙烯缝线修复，并覆盖一层涂层的聚丙烯（n = 20）、未涂层的聚丙烯（n = 18）或无网片（n = 16）。修复后 7 或 90 天采集标本进行组织学、免疫组织化学和信使核糖核酸（mRNA）分析。实时定量聚合酶链反应（PCR）芯片用于分析组织-网片界面 84 个基因的表达。
植入后 1 周，与未涂层的网片相比，涂层网片引起了稍强的炎症反应，并增加了 4 种促炎细胞因子的 mRNA 表达。然而，两种材料在植入后 90 天与缝线修复相比，均诱导了相似的细胞因子和基质金属蛋白酶的表达。
胶原蛋白涂层的聚丙烯网片在愈合早期比未涂层的网片引起更高的炎症细胞因子表达，但在植入后 90 天，两种网片的反应相似。
