Health Sciences Saitama Prefectural University, Japan.
Differentiation. 2011 Jun;81(5):299-306. doi: 10.1016/j.diff.2011.03.002. Epub 2011 Apr 16.
Tenascin C (TNC) is an extracellular glycoprotein that is thought to be involved in tissue remodeling during organogenesis and regeneration. Using avian embryonic hearts, we investigated the spatiotemporal expression patterns of TNC during the formation of the proximal coronary artery. Immunohistochemistry showed that TNC was deposited around the developing coronary stem and that TNC colocalized with vascular smooth muscle α-actin. A quail-chick chimera, in which a quail proepicardial organ (PEO) had been transplanted, showed that quail tissue-derived cells contributed to the establishment of the endothelial and mural cells of the proximal coronary artery, and the quail tissue-derived mural cells displayed TNC. Proepicardial cells cultured in TNC showed the myofibroblast/smooth muscle cell phenotype and neutralizing anti-TNC antibody suppressed the expression of smooth muscle markers. These observations suggest that TNC plays a role in the mural smooth muscle development of the nascent proximal coronary artery.
Tenascin C（TNC）是一种细胞外糖蛋白，被认为参与器官发生和再生过程中的组织重塑。我们使用禽类胚胎心脏研究了 TNC 在近端冠状动脉形成过程中的时空表达模式。免疫组织化学显示，TNC 沉积在发育中的冠状动脉干周围，并且 TNC 与血管平滑肌α-肌动蛋白共定位。鹌鹑-鸡嵌合体实验中，移植了一个鹌鹑的心包前体细胞（PEO），结果表明，来自鹌鹑组织的细胞有助于建立近端冠状动脉的内皮细胞和壁细胞，并且来自鹌鹑组织的壁细胞显示出 TNC。在 TNC 中培养的心包前体细胞表现出成纤维细胞/平滑肌细胞表型，中和抗 TNC 抗体抑制了平滑肌标志物的表达。这些观察结果表明，TNC 在初生近端冠状动脉的壁平滑肌发育中发挥作用。
