State Key Joint Laboratory of Environmental Stimulation and Pollution Control, School of Environment, Tsinghua University, Beijing, China.
Appl Environ Microbiol. 2011 Jun;77(12):4241-5. doi: 10.1128/AEM.01834-10. Epub 2011 Apr 15.
Three whole-community genome amplification methods, Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias than Bst. For community DNA, REPLI-g showed the least bias and highest number of genes, while Bst had the highest success rate and was suitable for low-quality DNA.
采用基于微阵列的方法评估了三种全社区基因组扩增方法，Bst、REPLI-g 和 Templiphi。所有方法的扩增偏差均<3 倍。对于纯培养 DNA，REPLI-g 和 Templiphi 的偏差小于 Bst。对于社区 DNA，REPLI-g 表现出最小的偏差和最多的基因，而 Bst 具有最高的成功率，并且适用于低质量的 DNA。
