Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
Appl Microbiol Biotechnol. 2011 Jul;91(2):287-94. doi: 10.1007/s00253-011-3267-6. Epub 2011 Apr 16.
A novel water-soluble dextran was synthesized from maltodextrin by cell-free extract of Gluconobacter oxydans DSM 2003. The dextran was purified by size exclusion chromatography, and the structure was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and gas chromatography-mass spectrometer. Based on the spectral data, we found that the dextran contained only D-glucose residues. The ratio of nonreducing end glucopyranosyl (Glcp) to 6-linked Glcp to 4,6-linked Glcp was estimated to be 8.62:78.79:12.59 by methylation analysis. This result indicated the existence of a small proportion of α(1,4) branches in α(1,6) glucosyl linear chains. Here, we reported the first time a novel dextran was synthesized by G. oxydans DSM 2003.
一种新型的水溶性葡聚糖是由氧化葡萄糖杆菌（Gluconobacter oxydans）DSM 2003 的无细胞提取物从麦芽糊精合成的。葡聚糖通过尺寸排阻色谱法进行纯化，并通过傅里叶变换红外光谱、核磁共振和气相色谱-质谱进行结构测定。根据光谱数据，我们发现葡聚糖仅含有 D-葡萄糖残基。通过甲基化分析，估计非还原端吡喃葡萄糖基（Glcp）与 6-连接的 Glcp 和 4,6-连接的 Glcp 的比例为 8.62:78.79:12.59。这一结果表明在α（1,6）葡糖基直链中存在少量的α（1,4）支链。在这里，我们首次报道了由氧化葡萄糖杆菌 DSM 2003 合成的新型葡聚糖。
