Division of Chemistry and Forensic Science, University of Glamorgan, CF37 4AT.
Drug Test Anal. 2011 Apr;3(4):201-5. doi: 10.1002/dta.172. Epub 2010 Oct 28.
A gas chromatographic tandem mass spectrometric (GCMS/MS) method for simultaneously determining trace concentrations of gamma-hydroxybutyrate (GHB) and ethyl glucuronide (EtG) in hair has been developed. Multiple reaction monitoring (MRM) was used to detect precursor and product ions of GHB, (233 and 147) and EtG (261 and 143) following anion exchange solid phase extraction and derivatization with N,O-bis[trimethylsilyl]trifluoroacetamide (BSTFA). Deuterated standards of GHB and EtG were used as internal standards. The assay produced excellent linearity (r(2) > 0.99) and sensitivity. The lower limit of quantitation (LLOQ) was 10 pg/mg for EtG assuming a 20 mg hair sample. The method has been used to investigate cases of suspected drug facilitated assault as well as being used to identify heavy alcohol consumption in a group of volunteers.
建立了一种气相色谱-串联质谱（GCMS/MS）方法，用于同时检测头发中痕量γ-羟基丁酸（GHB）和乙基葡糖苷酸（EtG）的浓度。采用多反应监测（MRM）模式，在阴离子交换固相萃取和 N,O-双[三甲基硅基]三氟乙酰胺（BSTFA）衍生化后，检测 GHB（233 和 147）和 EtG（261 和 143）的前体离子和产物离子。使用氘代 GHB 和 EtG 标准品作为内标。该测定方法具有极好的线性（r(2) > 0.99）和灵敏度。假设使用 20 毫克头发样本，EtG 的定量下限（LLOQ）为 10 pg/mg。该方法已用于调查疑似药物辅助攻击的案件，并用于鉴定一组志愿者中的大量饮酒情况。
