Liu Yu, Luo Chao-zhi
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Mar;42(2):231-3.
To investigate the protective effect and mechanisms of Fospropofol disodium on in vivo liver ischemia-reperfusion injury in rats.
Forty SD rats were randomly divided into 5 groups: sham operation group, NS pretreated group (NS), Fospropofol disodium pretreated group (SP), Propofol pretreated group (P) and 10% fat milk pretreated group (Z). After the hepatic inflow was occluded for 30 min and reperfused for 90 min, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were determined, and the liver specimen were obtained for the determination of superoxide dismutase (SOD) activities and malondialdehyde (MDA) contents and for microscopic examinations.
The serum levels of ALT, AST and LDH in the rats in group NS, SP, P, and Z after liver ischemia-reperfusion were significantly higher than those with sham operations (P < 0.01). The serum levels of ALT, AST and LDH in the rats in group SP, P, Z were significantly lower than those in group NS (P < 0.01). The MDA contents and SOD activities in the livers of rats in group NS, SP, P and Z were significantly higher than in those with sham operations (P < 0.01). The MDA contents and SOD activities in the livers of rats in group SP, P and Z were significantly lower than those in group NS (P < 0.01).
Fospropofol Disodium has a protective effect on rat livers against ischemia-reperfusion injury, which is related with antioxidation.
探讨磷丙泊酚二钠对大鼠体内肝脏缺血再灌注损伤的保护作用及机制。
将40只SD大鼠随机分为5组：假手术组、生理盐水预处理组（NS）、磷丙泊酚二钠预处理组（SP）、丙泊酚预处理组（P）和10%脂肪乳预处理组（Z）。肝门血流阻断30分钟后再灌注90分钟，测定血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LDH）水平，并取肝脏标本测定超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，进行显微镜检查。
肝脏缺血再灌注后，NS组、SP组、P组和Z组大鼠血清ALT、AST和LDH水平显著高于假手术组（P<0.01）。SP组、P组、Z组大鼠血清ALT、AST和LDH水平显著低于NS组（P<0.01）。NS组、SP组、P组和Z组大鼠肝脏MDA含量和SOD活性显著高于假手术组（P<0.01）。SP组、P组和Z组大鼠肝脏MDA含量和SOD活性显著低于NS组（P<0.01）。
磷丙泊酚二钠对大鼠肝脏缺血再灌注损伤具有保护作用，其机制与抗氧化作用有关。
