Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Cancer Biol Ther. 2011 Jun 1;11(11):974-80. doi: 10.4161/cbt.11.11.15705.
Cancer stem cells are defined as a subpopulation of cancer cells with the capacity to self-renew and differentiate, which may play critical roles in tumor initiation, progress and resistance to current treatments. It has been reported that Dendritic cells (DCs) transfected with total tumor RNA could induce strong antitumor T-cell responses both in vivo and in vitro. In the study, we investigated the characteristics of 9L tumor spheres, and evaluated the antitumor effects of DCs transfected with 9L tumor spheres RNA in vivo. The results showed that 9L tumor spheres have the properties of cancer stem cells, and the majority of 9L cells were positive for CD133 and nestin. DCs transfected with 9L tumor spheres RNA can significantly inhibit glioma growth and prolong the survival of 9L glioma-bearing rats. These results demonstrated that 9L cancer stem like cells were enriched in tumor spheres, and they were a part of CD133+ cells, DCs transfected with cancer stem cells RNA may be an effective therapy for glioma.
肿瘤干细胞被定义为具有自我更新和分化能力的肿瘤细胞亚群，可能在肿瘤的发生、进展和对现有治疗的耐药性中发挥关键作用。有报道称，用肿瘤总 RNA 转染的树突状细胞（DCs）可在体内和体外诱导强烈的抗肿瘤 T 细胞反应。在本研究中，我们研究了 9L 肿瘤球的特征，并评估了转染 9L 肿瘤球 RNA 的 DCs 在体内的抗肿瘤作用。结果表明，9L 肿瘤球具有肿瘤干细胞的特性，大多数 9L 细胞 CD133 和巢蛋白阳性。转染 9L 肿瘤球 RNA 的 DCs 可显著抑制神经胶质瘤的生长并延长 9L 神经胶质瘤荷瘤大鼠的生存期。这些结果表明，9L 类肿瘤干细胞样细胞在肿瘤球中富集，并且是 CD133+细胞的一部分，转染肿瘤干细胞 RNA 的 DCs 可能是一种有效的神经胶质瘤治疗方法。
