吡啶核苷酸对线粒体ATP酶合成及水解ATP的影响。

Effect of pyridine nucleotides on ATP synthesis and hydrolysis by the mitochondrial ATPase.

作者信息

Baizabal-Aguirre V M, Behrens M I, Gómez-Puyou A, Tuena de Gómez-Puyou M

机构信息

Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, D.F.

出版信息

Biochem Int. 1990 Nov;22(4):677-84.

PMID:2150308

Abstract

The effect of NADH, NAD+, NADPH, and NADP+ on succinate driven ATP synthesis of Mg-ATP submitochondrial particles and on ATP hydrolysis in soluble and particulate F1 from bovine heart was studied. Hydrolysis was inhibited competitively by all pyridine nucleotides when the concentration of Mg [gamma 32P]-ATP was varied between 0.1 mM to 3 mM, but NAD+ was less effective. Succinate driven ATP synthesis at various concentrations of ADP was also inhibited by the four pyridine nucleotides, but NADH was much more effective. Increasing concentrations of phosphate decreased the extent of the NADH induced inhibition of ATP synthesis. The effect of various parts of the NADH molecule on ATP synthesis was tested; the order of inhibition by these compounds was: NADH greater than ADP-ribose greater than NAD+ greater than NADPH greater than NADP+ greater than nicotinamide mononucleotide greater than nicotinamide.

摘要

研究了NADH、NAD⁺、NADPH和NADP⁺对琥珀酸驱动的Mg-ATP亚线粒体颗粒ATP合成以及对牛心可溶性和颗粒性F1中ATP水解的影响。当Mg[γ-32P]-ATP浓度在0.1 mM至3 mM之间变化时，所有吡啶核苷酸都竞争性抑制水解，但NAD⁺的效果较差。在不同浓度的ADP下，琥珀酸驱动的ATP合成也受到这四种吡啶核苷酸的抑制，但NADH的效果要强得多。磷酸盐浓度的增加降低了NADH诱导的ATP合成抑制程度。测试了NADH分子不同部分对ATP合成的影响；这些化合物的抑制顺序为：NADH＞ADP-核糖＞NAD⁺＞NADPH＞NADP⁺＞烟酰胺单核苷酸＞烟酰胺。