Shearwin K, Masters C
Division of Science and Technology, Griffith University, Nathan, Brisbane, Australia.
Biochem Int. 1990 Nov;22(4):735-40.
In a continuing study of the interactions between glycolytic enzymes and cytoskeletal structure, the influence of a variation of the pH of the eluting medium has been investigated. This treatment resulted in an increased degree of binding of most of the glycolytic enzymes with a decrease in pH, with the most marked increases in binding occurring with phosphofructokinase, glyceraldehydephosphate dehydrogenase, enolase and pyruvate kinase. The significance of this data has been discussed with reference to the relative affinities of interaction of the individual glycolytic components and the physiological correlations of these phenomena.
在一项关于糖酵解酶与细胞骨架结构相互作用的持续研究中，研究了洗脱介质pH值变化的影响。这种处理导致大多数糖酵解酶的结合程度随pH值降低而增加，其中磷酸果糖激酶、甘油醛 - 3 - 磷酸脱氢酶、烯醇化酶和丙酮酸激酶的结合增加最为显著。已参照各个糖酵解成分相互作用的相对亲和力以及这些现象的生理相关性对该数据的意义进行了讨论。
