Department of Chemistry, University of Cincinnati, 301 Clifton Court, Cincinnati, Ohio 45221-0172, USA.
Anal Chem. 2011 May 15;83(10):3725-9. doi: 10.1021/ac200161s. Epub 2011 Apr 19.
Spectroelectrochemical sensing in an optically transparent thin layer electrode (OTTLE) cell was used for detecting the polycyclic aromatic hydrocarbon (PAH) biomarkers 1-hydroxypyrene (1-pyOH) and 1-hydroxypyrene-glucuronide (1-pyOglu) in phosphate buffer and artificial urine. This approach uses selective electrochemical modulation of a fluorescence signal by sequentially oxidizing the analytes in an OTTLE cell to distinguish between their overlapping fluorescence spectra. This technique allows for complete oxidation and signal modulation in approximately 15 min for each analyte; a mixture of 1-pyOH and its glucuronic acid conjugate can be analyzed in 30 min. Calibration curves consisting of the fluorescence change vs analyte concentration for 1-pyOH and 1-pyOglu yielded linear ranges from 10 nM to 1 μM and from 1 nM to 1 μM, respectively. With the use of these results, the calculated limits of detection were determined to be 1 × 10(-8) M for 1-pyOH and 9 × 10(-11) M for 1-pyOglu.
在光学透明薄层电极 (OTTLE) 池中的光谱电化学传感用于检测多环芳烃 (PAH) 生物标志物 1-羟基芘 (1-pyOH) 和 1-羟基芘-葡糖苷酸 (1-pyOglu) 在磷酸盐缓冲液和人工尿液中的含量。该方法使用荧光信号的选择性电化学调制，通过依次在 OTTLE 池中将分析物氧化来区分它们重叠的荧光光谱。该技术允许每个分析物在大约 15 分钟内完成完全氧化和信号调制；可以在 30 分钟内分析 1-pyOH 和其葡萄糖醛酸缀合物的混合物。包含 1-pyOH 和 1-pyOglu 的荧光变化与分析物浓度的校准曲线分别产生了 10 nM 至 1 μM 和 1 nM 至 1 μM 的线性范围。根据这些结果，计算出 1-pyOH 的检测限为 1×10(-8) M，1-pyOglu 的检测限为 9×10(-11) M。
