Buckley M G, Sagnella G A, Markandu N D, Singer D R, MacGregor G A
Department of Medicine, Charing Cross and Westminister Medical School, London, England.
Am J Hypertens. 1990 Dec;3(12 Pt 1):933-5. doi: 10.1093/ajh/3.12.933.
N-Terminal pro ANP (atrial natriuretic peptide) in human plasma has been measured by radioimmunoassay after extraction on Sep-Pak cartridges. Immunoreactive N-terminal pro ANP circulates in human plasma at higher levels than alpha-hANP (approximately 20-fold higher in normal subjects) and was elevated in patients with essential hypertension, cardiac transplantation and patients with chronic renal failure. In chronic renal failure patients undergoing hemodialysis, C-terminal ANP (ANP 99-126), but not N-terminal ANP, declined significantly after dialysis. Gel filtration experiments demonstrated a single peak of N-terminal ANP immunoreactivity, eluting in parallel with synthetic human pro ANP 1-67, indicating a similar molecular size and the absence of low molecular weight N-terminal fragments.
用放射免疫分析法在Sep-Pak柱上萃取后测定人血浆中的N末端前心钠素（ANP）。免疫反应性N末端前心钠素在人血浆中的循环水平高于α-hANP（正常受试者中约高20倍），在原发性高血压患者、心脏移植患者和慢性肾衰竭患者中升高。在接受血液透析的慢性肾衰竭患者中，透析后C末端ANP（ANP 99-126）显著下降，但N末端ANP未下降。凝胶过滤实验显示N末端ANP免疫反应性有一个单一峰，与合成的人前ANP 1-67平行洗脱，表明分子大小相似且不存在低分子量N末端片段。
