Shrestha E, Shrestha J K, Shayami G, Chaudhary M
Consultant Ophthalmologist, Himalaya Eye Hospital, Pokhara, Nepal.
Nepal J Ophthalmol. 2011 Jan-Jun;3(1):39-44. doi: 10.3126/nepjoph.v3i1.4277.
The dry eye or tear film dysfunction is a common ophthalmic syndrome.
To compare the results of conjunctival impression cytology between dry eye patients and normal individuals.
A case control study including consecutive cases of dry eye syndrome was carried out. Individuals without dry eye were taken as control. Impression of conjunctiva with cellulose acetate filter paper was taken from inferonasal bulbar conjunctiva and was stained with Periodic Acid- Schiff (PAS) and counter-stained with haematoxylin and eosin.
goblet cell density.
There was a female preponderance in dry eye disease. Of 114 dry eye cases, 49.2% eyes showed decreased or absent goblet cell density. In 72 normal individuals 73.7% eyes showed normal goblet cell density and 26.3% of eyes showed decreased or absent goblet cells (p less than 0.001). The tear break-up time (TBUT) test was significantly more likely to be less than 10 seconds in cases as compared to the controls ( OR = 19.36, 95% CI = 7.56 - 52.52). Similarly, the goblet cell density was likely to be significantly reduced in cases with dry eye syndrome (OR= 2.25, 95% CI = 1.26 - 4.02, p = 0.003).
Goblet cell density significantly reduces in dry eye syndrome. The impression cytology is a useful test for the diagnosis of dry eye syndrome.
干眼或泪膜功能障碍是一种常见的眼科综合征。
比较干眼患者与正常个体结膜印迹细胞学检查结果。
进行一项病例对照研究，纳入连续的干眼综合征病例。无干眼的个体作为对照。用醋酸纤维素滤纸从鼻下球结膜取结膜印迹，用高碘酸-希夫（PAS）染色，苏木精和伊红复染。
杯状细胞密度。
干眼疾病中女性占优势。114例干眼病例中，49.2%的眼杯状细胞密度降低或缺失。72例正常个体中，73.7%的眼杯状细胞密度正常，26.3%的眼杯状细胞密度降低或缺失（p<0.001）。与对照组相比，病例组泪膜破裂时间（TBUT）试验显著更易小于10秒（OR = 19.36，95%CI = 7.56 - 52.52）。同样，干眼综合征病例杯状细胞密度可能显著降低（OR = 2.25，95%CI = 1.26 - 4.02，p = 0.003）。
干眼综合征中杯状细胞密度显著降低。印迹细胞学检查是诊断干眼综合征的一种有用检查。
