Martinez I, Ofstad R, Olsen R L
Institute of Fisheries Technology Research, Tromsø, Norway.
J Muscle Res Cell Motil. 1990 Dec;11(6):489-95. doi: 10.1007/BF01745216.
The myosin content from red and white muscles of three marine fish species, saithe (Pollachius virens. L.), haddock (Melanogrammus aeglefinus, L.), both members of the family Gadidae, and capeline (Mallotus villosus, M.) of the family Osmeridae, was analyzed electrophoretically. Analysis of the native myosin by electrophoresis under non-dissociating conditions revealed two isoforms in red muscles, and three or four in white muscles. The white muscles of the two closely related species had a similar pattern of isoforms. Myosin from the slow red muscles had two types of light chain, LC1S and LC2S, and myosin from the fast white muscles three, LC1F, LC2F, and LC3F. The pattern of light chains in both types of muscles was species-dependent. All the light chains from fish myosins were more acidic than those of the rat diaphragm used as standard. One main type of heavy chain was detected in each kind of muscle. In white muscles of saithe there was an extra band, present in minor amounts. The heavy chains from white muscle myosin had lower electrophoretic mobilities than those from red muscle, and the mobilities of all of them were intermediate between those of the heavy chains type IIa and I of rat diaphragm myosin. In our opinion, there are probably more isomyosins in fish muscles than those detected in the present work and their presence is obscured by comigration with the main types.
对三种海鱼（鳕鱼科的绿青鳕（Pollachius virens. L.）和黑线鳕（Melanogrammus aeglefinus, L.）以及胡瓜鱼科的毛鳞鱼（Mallotus villosus, M.））红肌和白肌中的肌球蛋白含量进行了电泳分析。在非解离条件下对天然肌球蛋白进行电泳分析发现，红肌中有两种同工型，白肌中有三种或四种同工型。这两种亲缘关系密切的物种的白肌具有相似的同工型模式。慢红肌中的肌球蛋白有两种轻链类型，即LC1S和LC2S，快白肌中的肌球蛋白有三种轻链类型，即LC1F、LC2F和LC3F。两种类型肌肉中的轻链模式因物种而异。鱼类肌球蛋白的所有轻链都比用作标准的大鼠膈肌的轻链酸性更强。在每种肌肉中都检测到一种主要类型的重链。在绿青鳕的白肌中有一条额外的条带，含量较少。白肌肌球蛋白的重链电泳迁移率低于红肌中的重链，且所有重链的迁移率介于大鼠膈肌肌球蛋白重链IIa型和I型之间。我们认为，鱼类肌肉中可能存在比本研究中检测到的更多的同功肌球蛋白，它们的存在因与主要类型的共迁移而被掩盖。
