Gerhold Richard W, Lollis Lori A, McDougald Larry R, Beckstead Robert B
Department of Poultry Science, University of Georgia, Athens, Georgia 30602, USA.
J Parasitol. 2011 Apr;97(2):354-6. doi: 10.1645/GE-2618.1. Epub 2010 Oct 27.
Histomonas meleagridis , the causative agent of histomoniasis, is a protozoan parasite classified in the Dientamoebidae (order Tritrichomonadida). The α-tubulin gene of 7 H. meleagridis isolates originating from either domestic chickens or turkeys from the United States was amplified by nested PCR and sequenced. A 91.4-99.8% nucleotide identity was shared among the 7 different sequences, and phylogenetic analysis disclosed that the 7 isolates were divided into at least 3 clades. These sequences had a 91-99% nucleotide identity and a 96-100% amino acid identity compared with 3 H. meleagridis α-tubulin sequences obtained from isolates originating from turkeys in Germany. Further α-tubulin gene analysis from species in the Dientamoebidae will be useful in elucidating the evolutionary relationship of these protozoans.
火鸡组织滴虫是组织滴虫病的病原体，是一种分类在双滴虫科（三毛滴虫目）的原生动物寄生虫。通过巢式PCR扩增并测序了来自美国家鸡或火鸡的7株火鸡组织滴虫分离株的α-微管蛋白基因。7个不同序列之间的核苷酸同一性为91.4%-99.8%，系统发育分析表明这7个分离株至少分为3个进化枝。与从德国火鸡分离株获得的3个火鸡组织滴虫α-微管蛋白序列相比，这些序列的核苷酸同一性为91%-99%，氨基酸同一性为96%-100%。对双滴虫科物种的进一步α-微管蛋白基因分析将有助于阐明这些原生动物的进化关系。
