Hauck Rüdiger, Balczulat Stefanie, Hafez Hafez M
Institute for Poultry Diseases, Free University Berlin, Koenigsweg 63, 14163 Berlin, Germany.
Avian Dis. 2010 Sep;54(3):1021-5. doi: 10.1637/9261-012910-Reg.1.
Between 2004 and 2008, 338 samples from 156 German turkey, chicken, and peacock flocks with suspected histomonosis (histomoniasis) were sent to the Institute for Poultry Diseases of the Free University Berlin. Most samples were from ceca or livers; the other samples were organ pools or were taken from other organs or the environment. In 108 samples from 65 flocks, histomonal DNA was detected by polymerase chain reaction (PCR). Tetratrichomonas gallinarum DNA was found in 5.3% of investigated samples from flocks infected with Histomonas meleagridis and in 27.4% of investigated samples from flocks that were not infected with H. meleagridis. For subtyping of the strains, the C-profiling method, a method used to analyze the internal transcribed spacer-1 (ITS-1) of the rRNA gene, was modified to be more specific for H. meleagridis. Results showed the presence of more than the three subtypes described so far. There was no clear correlation between the subtype and the host. By C-profiling the clonal cultures, heterogeneous ITS-1 sequences were shown to probably result from intragenomic differences between rRNA genes.
2004年至2008年间，来自156个德国火鸡、鸡和孔雀群且疑似感染组织滴虫病的338份样本被送往柏林自由大学家禽疾病研究所。大多数样本取自盲肠或肝脏；其他样本为器官混合样本或取自其他器官或环境。在来自65个鸡群的108份样本中，通过聚合酶链反应（PCR）检测到组织滴虫DNA。在感染火鸡组织滴虫的鸡群的5.3%的检测样本以及未感染火鸡组织滴虫的鸡群的27.4%的检测样本中发现了鸡四毛滴虫DNA。为了对菌株进行亚型分析，对用于分析rRNA基因内部转录间隔区1（ITS-1）的C-分型方法进行了改进，使其对火鸡组织滴虫更具特异性。结果显示存在超过目前已描述的三种亚型。亚型与宿主之间没有明显的相关性。通过对克隆培养物进行C-分型，结果表明ITS-1序列的异质性可能是由rRNA基因之间的基因组内差异导致的。
