中国河北省长角血蜱中斑点热群立克次体的检测。
Detection of spotted fever group Rickettsia in Haemaphysalis longicornis from Hebei Province, China.
作者信息
Zou Yaxue, Wang Qiuyue, Fu Zhixin, Liu Pengpeng, Jin Hongtao, Yang Huanhuan, Gao Hongwei, Xi Zhang, Liu Quan, Chen Lifeng
机构信息
Key Laboratory of Preventive Veterinary Medicine in Hebei Province, Hebei Normal University of Science & Techology, 360 Hebei Street, Qinhuangdao, 066000, China.
出版信息
J Parasitol. 2011 Oct;97(5):960-2. doi: 10.1645/GE-2751.1. Epub 2011 Mar 23.
DNA samples from 737 tick pools, representing 6,850 Haemaphysalis longicornis and 51 Dermacentor nuttalli collected from Hebei Province, China, were analyzed by polymerase chain reaction (PCR) for the presence of spotted fever group Rickettsia. Fifty (6.9%) of 724 H. longicornis in the tick pool were positive, but no positive samples were found in 13 D. nuttalli. Sequence analysis of the partial outer membrane protein A (ompA) genes from the 10 positive samples showed 97.4-99.8% identity, but were different from the homologous sequence of Rickettsia previously deposited in GenBank. Phylogenetic analysis of ompA genes indicated that the Rickettsia detected in this study belonged to a novel haplotype, and formed a clade distinct from Rickettsia heilongjiangii, Rickettsia sibirica, and Rickettsia hulinii in China. The new strain, named Candidatus Rickettsia hebeiii, appears to represent a distinct lineage and could constitute a new species with a minimum prevalence of about 0.7% in H. longicornis from Hebei Province, China.
从中国河北省采集的737个蜱虫样本池中的DNA进行分析，这些样本池代表了6850只长角血蜱和51只草原革蜱，采用聚合酶链反应（PCR）检测斑点热群立克次体的存在。蜱虫样本池中724只长角血蜱中有50只（6.9%）呈阳性，但13只草原革蜱中未发现阳性样本。对10个阳性样本的部分外膜蛋白A（ompA）基因进行序列分析，结果显示同一性为97.4 - 99.8%，但与之前存于GenBank中的立克次体同源序列不同。ompA基因的系统发育分析表明，本研究中检测到的立克次体属于一个新的单倍型，并且形成了一个与中国的黑龙江立克次体、西伯利亚立克次体和虎林立克次体不同的分支。这个新菌株命名为“候选立克次体河北株（Candidatus Rickettsia hebeiii）”，似乎代表一个独特的谱系，并且可能构成一个新物种，在中国河北省长角血蜱中的最低流行率约为0.7%。
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