Garoui El Mouldi, Troudi Afef, Fetoui Hamadi, Soudani Nejla, Boudawara Tahia, Zeghal Najiba
Animal Physiology Laboratory, U/R 08-73, Sciences Faculty, BP 1171, 3000 Sfax, University of Sfax, Tunisia.
Exp Toxicol Pathol. 2012 Nov;64(7-8):837-46. doi: 10.1016/j.etp.2011.03.004. Epub 2011 Apr 19.
The aim of this study was to evaluate the biochemical changes in cobalt-exposed rats and to investigate the potential role of Tunisian propolis against the cobalt-induced renal damages. Twenty-four pregnant Wistar rats were divided into four groups and were treated as follows: group 1 (control) received distilled water; group 2 received 350 ppm of CoCl(2) in drinking water; group 3 received 350 ppm CoCl(2) in drinking water and a propolis-supplemented diet (1 g/100 g of diet); group 4 received a propolis-supplemented diet (1 g/100 g of diet) without cobalt. In the cobalt group, a significant decrease in body, absolute and relative weights was noted when compared to controls. The administration of cobalt to pregnant rats from the 14th day of pregnancy until day 14 after delivery resulted in an increased level of renal malondialdehyde, a decreased renal content of glutathione and antioxidant enzyme activities such as superoxide dismutase, catalase and glutathione peroxidase in lactating rats and their pups. A statistically significant increase in plasma urea and creatinine serum levels was seen in treated female rats and their pups. Histopathologically, the cobalt-administration induced degenerative changes in the kidney of lactating rats and their pups. When compared with cobalt-treated rats, those receiving the propolis supplementation (along with cobalt-treatment) had lower malondialdehyde levels, higher antioxidant activities and the cobalt-related histopathological changes in the kidneys were at lower severity. Our results suggested that the propolis might be a potential candidate agent against cobalt-induced nephrotoxicity in adult and juvenile rats when administered to female rats during the late pregnancy and the early postnatal period.
本研究的目的是评估钴暴露大鼠的生化变化，并研究突尼斯蜂胶对钴诱导的肾损伤的潜在作用。将24只怀孕的Wistar大鼠分为四组，并进行如下处理：第1组（对照组）给予蒸馏水；第2组给予饮用水中含350 ppm的CoCl₂；第3组给予饮用水中含350 ppm CoCl₂以及补充蜂胶的饲料（1 g/100 g饲料）；第4组给予补充蜂胶的饲料（1 g/100 g饲料）但不含钴。与对照组相比，钴组大鼠的体重、绝对体重和相对体重均显著下降。从怀孕第14天到产后第14天给怀孕大鼠施用钴，导致哺乳期大鼠及其幼崽的肾丙二醛水平升高、肾谷胱甘肽含量降低以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶等抗氧化酶活性降低。在接受处理的雌性大鼠及其幼崽中，血浆尿素和血清肌酐水平有统计学意义的显著升高。组织病理学检查显示，施用钴会导致哺乳期大鼠及其幼崽的肾脏发生退行性变化。与钴处理的大鼠相比，接受补充蜂胶（与钴处理同时进行）的大鼠丙二醛水平较低、抗氧化活性较高，并且肾脏中与钴相关的组织病理学变化严重程度较低。我们的结果表明，在妊娠后期和产后早期给雌性大鼠施用蜂胶，可能是对抗成年和幼年大鼠钴诱导的肾毒性的潜在候选药物。
