Koizumi H, Tanaka H, Fukaya T, Yasui C, Ohkawara A
Department of Dermatology, Hokkaido University School of Medicine, Sapporo, Japan.
J Dermatol Sci. 1990 Jul;1(4):297-302. doi: 10.1016/0923-1811(90)90123-u.
The activation of protein kinase C involves its translocation from a cytosol fraction to a membrane fraction. Effects of interferon-gamma (IFN-gamma) on the epidermal protein kinase C were investigated. The treatment of recombinant human IFN-gamma on intact human epidermis resulted in the translocation of protein kinase C from a cytosol to a membrane fraction. The human IFN-gamma had no translocation effect on pig epidermal protein kinase C. Tumor promoter, 12-o-tetradecanoylphorbol-13-acetate (TPA), and a membrane-permeable diacylglycerol analogue, 1-oleoyl-2-acetylglycerol (OAG), both of which are well-known activators of protein kinase C, translocated the epidermal protein kinase C. The IFN-gamma had no direct effect on the epidermal protein kinase C; the addition of the IFN-gamma to partially-purified pig epidermal protein kinase C had no effect on its activity. The effect of the IFN-gamma on human epidermal protein kinase C appears to be through the species specific IFN-gamma receptors. It has been reported that the epidermal beta-adrenergic adenylate cyclase response is decreased following the TPA- (and OAG-) induced activation of protein kinase C. Human recombinant IFN-gamma, however, had no effect on the beta-adrenergic response of the human epidermis. Our results indicate that IFN-gamma affects intact keratinocytes in vitro, resulting in the activation of protein kinase C, which might be related to the physiological effect of IFN-gamma on keratinocyte.
蛋白激酶C的激活涉及到它从胞浆组分向膜组分的转位。研究了γ-干扰素（IFN-γ）对表皮蛋白激酶C的影响。用重组人IFN-γ处理完整的人表皮，导致蛋白激酶C从胞浆转位至膜组分。人IFN-γ对猪表皮蛋白激酶C没有转位作用。肿瘤启动子12-O-十四烷酰佛波醇-13-乙酸酯（TPA）和一种膜通透性二酰基甘油类似物1-油酰-2-乙酰甘油（OAG），二者均为蛋白激酶C的著名激活剂，均可使表皮蛋白激酶C发生转位。IFN-γ对表皮蛋白激酶C没有直接作用；向部分纯化的猪表皮蛋白激酶C中添加IFN-γ对其活性没有影响。IFN-γ对人表皮蛋白激酶C的作用似乎是通过物种特异性的IFN-γ受体实现的。据报道，在TPA（和OAG）诱导的蛋白激酶C激活后，表皮β-肾上腺素能腺苷酸环化酶反应会降低。然而，人重组IFN-γ对人表皮的β-肾上腺素能反应没有影响。我们的结果表明，IFN-γ在体外影响完整的角质形成细胞，导致蛋白激酶C激活，这可能与IFN-γ对角质形成细胞的生理作用有关。
