Chen Ren-Jin, Yang Zhang-Ping, Mao Yong-Jiang, Chen Ying, Chang Ling-Ling, Ji De-Jun, Wu Hai-Tao, Li Yun-Long, Li Rui
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Yi Chuan. 2010 Dec;32(12):1256-62.
The polymorphism of Interleukin-8 (IL8) gene were investigated for 610 Chinese Holstein cows of 30 bull families from a dairy farm in Shanghai using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) technique with a mixed animal model to verify the effects of the polymorphisms on some milk productive performance, tested day milk yield, tested day fat percentage, tested day milk protein percentage, 305 d corrected milk yield, 305 d milk fat yield, 305 d milk protein yield, and somatic cell score (SCS). The aim was to explore the significant molecular marker in practical dairy production. Three genotypes were identified and the genotypic frequencies of KK, KA, and AA were 0.187, 0.451, and 0.362, respectively. The gene frequencies of K and A were 0.412 and 0.588. The results showed highly significant (P < 0.01) association of IL8 mutations with tested day milk yield, 305 d milk protein yield, 305 d corrected milk yield and 305 d milk fat yield, SCS and tested day milk protein percentage (P < 0.05). However, no association (P > 0.05) with tested day milk fat percentage was recorded. The cows with KK genotype had higher tested day milk yield, 305 d milk protein yield, 305 d corrected milk yield and 305 d milk fat yield than those with AA and KA genotypes (P < 0.01). The least square mean of SCS for KK was significantly lower than that with AA and KA genotypes (P < 0.01). AA genotype was significant lower in tested day milk protein percentage than KK and KA genotypes (P < 0.05). The IL8 gene genetic diversity has a great genetic effect on milk traits and mastitis resistance and could be a useful genetic marker for Chinese Holstein breeding.
采用聚合酶链反应-单链构象多态性（PCR-SSCP）技术及混合动物模型，对上海某奶牛场30个公牛家系的610头中国荷斯坦奶牛白细胞介素8（IL8）基因的多态性进行了研究，以验证该基因多态性对部分产奶性能、测定日产奶量、测定日乳脂率、测定日乳蛋白率、305天校正产奶量、305天乳脂产量、305天乳蛋白产量及体细胞评分（SCS）的影响。目的是在实际奶牛生产中探索显著的分子标记。共鉴定出三种基因型，KK、KA和AA的基因型频率分别为0.187、0.451和0.362。K和A的基因频率分别为0.412和0.588。结果表明，IL8突变与测定日产奶量、305天乳蛋白产量、305天校正产奶量、305天乳脂产量、SCS及测定日乳蛋白率高度显著相关（P<0.01），与测定日乳脂率无显著相关性（P>0.05）。KK基因型奶牛的测定日产奶量、305天乳蛋白产量、305天校正产奶量和305天乳脂产量均高于AA和KA基因型奶牛（P<0.01）。KK基因型的SCS最小二乘均值显著低于AA和KA基因型（P<0.01）。AA基因型的测定日乳蛋白率显著低于KK和KA基因型（P<0.05）。IL8基因遗传多样性对奶牛产奶性状和抗乳腺炎能力具有重要遗传效应，可作为中国荷斯坦奶牛育种的有用遗传标记。
