Grimaudo V, Bachmann F, Kruithof E K
Department of Medicine, University Hospital Center, Lausanne, Switzerland.
Haemostasis. 1990;20(6):329-33. doi: 10.1159/000216146.
To estimate the extent of the release of plasminogen activator inhibitor type 1 (PAI-1) from platelets following their accidental activation in vitro during blood collection, we studied the antigen levels of PAI-1 and beta-thromboglobulin (beta TG) in plasma and sera from 38 healthy volunteers. For each volunteer, we calculated the ratio of platelet-derived PAI-1/platelet-derived beta TG from the difference in their respective concentrations in serum and plasma. The mean +/- SD (0.012 +/- 0.003) and median (0.012) ratios were identical before and after 10 or 20 min of venous occlusion. The platelet contribution of PAI-1 in plasma was estimated by multiplying the plasma concentration of beta TG (as a marker of platelet activation) with the above-mentioned individual ratio. Under our conditions of blood collection (1/10 vol of 0.1 M citrate, pH 4.5, as anticoagulant, and handling of blood at 4 degrees C and within 30 min), the platelet contribution to the PAI-1 concentration in plasma was on average 12% and always negligible in plasma with high levels of PAI-1.
为了评估在血液采集过程中血小板意外体外激活后纤溶酶原激活物抑制剂1（PAI - 1）的释放程度，我们研究了38名健康志愿者血浆和血清中PAI - 1及β - 血小板球蛋白（βTG）的抗原水平。对于每位志愿者，我们根据血清和血浆中各自浓度的差异计算血小板源性PAI - 1/血小板源性βTG的比值。静脉闭塞10或20分钟前后，平均±标准差（0.012±0.003）及中位数（0.012）比值相同。通过将血浆中βTG的浓度（作为血小板激活的标志物）与上述个体比值相乘来估算血浆中PAI - 1的血小板贡献。在我们的血液采集条件下（使用1/10体积的0.1M柠檬酸盐，pH 4.5作为抗凝剂，并在4℃下30分钟内处理血液），血小板对血浆中PAI - 1浓度的贡献平均为12%，在PAI - 1水平较高的血浆中始终可忽略不计。
