Dirks Wilhelm G, Drexler Hans G
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Methods Mol Biol. 2011;731:45-55. doi: 10.1007/978-1-61779-080-5_5.
The main prerequisition for any research, development, or production programs involving cell lines is whether a cell line is authentic or not. Microsatellites in the human genome harboring short tandem repeat (STR) DNA markers allow the identification of individual cell lines at the DNA level. Polymerase chain reaction (PCR) amplification of eight highly polymorphic microsatellite STR loci and gender determination have been proven to be the best tools for screening the uniqueness of DNA profiles in an STR database. The main Biological Resource Centers (BRCs), ATCC, DSMZ, JCRB, and RIKEN, have generated large databases of STR cell line profiles for identity control. In cooperation with the Japanese BRCs, DSMZ has piloted the generation of the most comprehensive international reference database, which is linked to a simple search engine for interrogating STR cell line profiles. The tool of online -verification of cell line identities is available on the respective homepages of JCRB and DSMZ ( http://cellbank.nibio.go.jp/cellbank_e.html , http://www.dsmz.de/STRanalysis ). The following sections describe a rapid, practical, inexpensive, and reliable method available to students, technicians, and scientists.
任何涉及细胞系的研究、开发或生产项目的主要前提条件是细胞系是否真实。人类基因组中含有短串联重复（STR）DNA标记的微卫星能够在DNA水平上鉴定单个细胞系。对八个高度多态性微卫星STR位点进行聚合酶链反应（PCR）扩增以及性别鉴定，已被证明是在STR数据库中筛选DNA图谱独特性的最佳工具。主要的生物资源中心（BRCs），如美国典型培养物保藏中心（ATCC）、德国微生物和细胞培养物保藏中心（DSMZ）、日本细胞库（JCRB）和日本理化学研究所生物资源中心（RIKEN），已经建立了大型的STR细胞系图谱数据库用于身份验证。DSMZ与日本的生物资源中心合作，试点建立了最全面的国际参考数据库，该数据库与一个用于查询STR细胞系图谱的简单搜索引擎相连。细胞系身份在线验证工具可在JCRB和DSMZ各自的主页上获取（http://cellbank.nibio.go.jp/cellbank_e.html，http://www.dsmz.de/STRanalysis）。以下部分介绍一种可供学生、技术人员和科学家使用的快速、实用、廉价且可靠的方法。
