Campa-Córdova Angel Isidro, Luna-González Antonio, Mazón-Suastegui José Manuel, Aguirre-Guzmán Gabriel, Ascencio Felipe, González-Ocampo Héctor Abelardo
Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Sinaloa, Boulevard Juan de Dios Bátiz Paredes 252, Col. San Joachin, C.P. 81101, Guasave, Sinaloa, México.
Rev Biol Trop. 2011 Mar;59(1):183-91.
Disease control problems have major constraints in aquaculture production, and the use of probiotics in larviculture is a valid alternative to antibiotics. This study analyzed the effect of probiotic bacteria on survival and final size of Cortez oyster larvae Crassostrea corteziensis. Two different probiotic concentrations were evaluated, 1 x 10(4) and 1 x 10(5) CFU/ml of Lactic acid bacteria (strain NS61) isolated from Nodipecten subnodosus, and bacilli isolated from the white leg shrimp, Litopenaeus vannamei (Pseudomonas aeruginosa, strain YC58) and C. corteziensis (Burkholderia cepacia, strain Y021). Bacteria were added directly into culture tanks, starting the bioassays from veliger to pediveliger stages as follows: (1) Control, without probiotics; (2) lactic acid bacteria (Lb); (3) bacilli mix (Mb) in a proportion 1:1. Results showed a higher larval survival with Lb and Mb at a dose of 1 x 10(4) CFU/ml compared to the control group. Larvae exposed to Mb at 1 x 10(5) CFU/ml showed higher survival than Lb and control. Larval final size was not significantly increased with the tested probiotics, but larvae treated with Lb at 1 x 10(5) CFU/ml showed less survival rate than those treated at 1 x 10(4) CFU/ml. This study showed the beneficial effect of these probiotics, added individually or mixed in C. corteziensis larvae culture.
疾病控制问题是水产养殖生产中的主要制约因素，在幼体养殖中使用益生菌是抗生素的有效替代品。本研究分析了益生菌对科尔特斯牡蛎幼虫（Crassostrea corteziensis）存活和最终大小的影响。评估了两种不同的益生菌浓度，即从结节栉孔扇贝（Nodipecten subnodosus）分离的乳酸菌（菌株NS61）、从凡纳滨对虾（Litopenaeus vannamei）分离的芽孢杆菌（铜绿假单胞菌，菌株YC58）和从科尔特斯牡蛎分离的洋葱伯克霍尔德菌（菌株Y021），浓度分别为1×10⁴和1×10⁵CFU/ml。将细菌直接添加到养殖池中，从面盘幼虫期到足面盘幼虫期开始进行生物测定，如下：（1）对照，不添加益生菌；（2）乳酸菌（Lb）；（3）芽孢杆菌混合物（Mb），比例为1:1。结果表明，与对照组相比，剂量为1×10⁴CFU/ml的Lb和Mb组幼虫存活率更高。暴露于1×10⁵CFU/ml Mb的幼虫存活率高于Lb组和对照组。经测试的益生菌未显著增加幼虫的最终大小，但用1×10⁵CFU/ml Lb处理的幼虫存活率低于用1×10⁴CFU/ml处理的幼虫。本研究表明，这些益生菌单独添加或混合添加到科尔特斯牡蛎幼虫养殖中均具有有益效果。
