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[树蛙(Smilisca baudinii,无尾目:雨蛙科)的减数分裂染色体]

[Meiotic chromosomes of the tree frog Smilisca baudinii (Anura: Hylidae)].

作者信息

Hernández-Guzmán Javier, Arias-Rodriguez Lenin, Indy Jeane Rimber

机构信息

División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, México.

出版信息

Rev Biol Trop. 2011 Mar;59(1):355-62.

Abstract

The Mexican tree frog Smilisca baudinii, is a very common frog in Central America. In spite their importance to keep the ecological equilibrium of the rainforest, its biology and genetics are poorly known. In order to contribute with its biological knowledge, we described the typical meiotic karyotype based in standard cytogenetic protocols to specimens collected in Tabasco, Mexico. The study was centered in the analysis of 131 chromosome spreads at meiotic stage from two adults of the species (one female and one male). The metaphase analysis allowed the establishment of the modal haploid number of 1n = 12 bivalent chromosomes. The chromosomic formulae from the haploid bivalent karyotype was integrated by 12 biarmed chromosomes characterized by twelve pairs of metacentric-submetacentric (msm) chromosomes. The meiotic counting gives the idea that diploid chromosome number is integrated by a complement of 2n = 24 biarmed chromosomes. The presence of sex chromosomes from female and male meiotic spreads was not observed. Current results suggest that S. baudinii chromosome structure is well shared among Hylidae family and "B" chromosomes are particular structures that have very important evolutionary consequences in species diversification.

摘要

墨西哥树蛙(Smilisca baudinii)是中美洲一种非常常见的蛙类。尽管它们对于维持雨林的生态平衡很重要,但其生物学和遗传学却鲜为人知。为了丰富对其生物学的认识,我们基于标准细胞遗传学方案,对在墨西哥塔巴斯科采集的标本描述了典型的减数分裂核型。该研究主要分析了该物种两只成年个体(一雌一雄)减数分裂阶段的131个染色体铺片。中期分析确定了单倍体模式数为1n = 12条二价染色体。单倍体二价核型的染色体公式由12条双臂染色体组成,其特征为十二对中着丝粒 - 亚中着丝粒(msm)染色体。减数分裂计数表明二倍体染色体数由2n = 24条双臂染色体组成。在雌性和雄性减数分裂铺片中未观察到性染色体的存在。目前的结果表明,鲍氏树蛙的染色体结构在雨蛙科中具有广泛的共性,而“B”染色体是特殊结构,在物种多样化过程中具有非常重要的进化意义。

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