Zhu Xiaoqin, Zhuo Shuangmu, Zheng Liqin, Jiang Xingshan, Chen Jianxin, Lin Bifang
Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
Scanning. 2011 Jul-Aug;33(4):195-200. doi: 10.1002/sca.20230. Epub 2011 Apr 21.
Multiphoton microscopy (MPM) was applied to examine the marginal region at dermis of keloid compared with atrophic scar. High-resolution large-area image showed an obvious boundary at the scar margin and different morphological patterns of elastin and collagen on the two sides, further visualized by the focused three-dimensional images. Content alteration of elastin or collagen between the two sides of boundary was quantified to show significant difference between keloid and atrophic scar. Owing to the raised property of keloid with overproduced collagen on the scar side, the content alteration was positive for elastin and negative for collagen. On the contrary, the content alteration was negative for elastin and positive for collagen in the atrophic scar case due to the atrophic collagen on the scar side. It indicated that examination of the scar margin by MPM may lead a new way to discriminate different types of scars and better understand the scarring mechanisms.
采用多光子显微镜（MPM）对瘢痕疙瘩真皮边缘区域与萎缩性瘢痕进行检查。高分辨率大面积图像显示瘢痕边缘有明显边界，两侧弹性蛋白和胶原蛋白呈现不同形态模式，聚焦三维图像进一步显示了这些情况。对边界两侧弹性蛋白或胶原蛋白的含量变化进行定量分析，结果显示瘢痕疙瘩与萎缩性瘢痕之间存在显著差异。由于瘢痕疙瘩隆起，瘢痕侧胶原蛋白过度产生，弹性蛋白含量变化为正，胶原蛋白含量变化为负。相反，在萎缩性瘢痕病例中，由于瘢痕侧胶原蛋白萎缩，弹性蛋白含量变化为负，胶原蛋白含量变化为正。这表明，利用MPM检查瘢痕边缘可能为鉴别不同类型瘢痕及更好地理解瘢痕形成机制开辟一条新途径。
