Masuda Minoru, Ohyama Kazutoshi, Hayashi Osamu, Satsuma Koji, Sato Kiyoshi
The Institute of Environmental Toxicology, Laboratory of Metabolism, Joso-shi, Japan.
Xenobiotica. 2011 Sep;41(9):818-25. doi: 10.3109/00498254.2011.574164. Epub 2011 Apr 27.
To obtain basic information on the metabolic fate of xenobiotics in the brackish water, bivalve Corbicula japonica, bioconcentration and biotransformation experiments were performed using methoxychlor (MXC) as a model compound. Bivalves were exposed to [ring-U-¹⁴C]MXC (10 µg L⁻¹) for 28 days under semi-static conditions followed by a 14-day depuration phase. The ¹⁴C concentration in the bivalves rapidly increased and reached a steady state after exposure for 7 days (BCFss = 2010); however, it rapidly decreased with a half-life of 2.2 days in the depuration phase. Mono- and bis-demethylated MXC, and their corresponding sulphate conjugates, were identified as minor metabolites. No glycoside conjugates (including glucuronide and glucoside) were detected. Despite this biotransformation system, bivalves were found to excrete retained MXC mostly unchanged although its relatively hydrophobic nature.
为获取有关半咸水双壳贝类日本蚬体内异生素代谢归宿的基础信息，以甲氧滴滴涕（MXC）作为模型化合物进行了生物富集和生物转化实验。双壳贝类在半静态条件下暴露于[环-U-¹⁴C]MXC（10 µg L⁻¹）28天，随后进入14天的净化阶段。双壳贝类体内的¹⁴C浓度迅速增加，暴露7天后达到稳态（BCFss = 2010）；然而，在净化阶段其迅速下降，半衰期为2.2天。单去甲基和双去甲基MXC及其相应的硫酸盐共轭物被鉴定为次要代谢产物。未检测到糖苷共轭物（包括葡糖醛酸和葡糖苷）。尽管存在这种生物转化系统，但发现双壳贝类排泄保留的MXC时大多未发生变化，尽管其具有相对疏水的性质。
