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1
A DNA-binding protein factor recognizes two binding domains within the octopine synthase enhancer element.一种DNA结合蛋白因子可识别章鱼碱合酶增强子元件内的两个结合结构域。
Plant Cell. 1990 Mar;2(3):215-24. doi: 10.1105/tpc.2.3.215.
2
Multiple ocs-like elements required for efficient transcription of the mannopine synthase gene of T-DNA in maize protoplasts.玉米原生质体中T-DNA甘露碱合成酶基因高效转录所需的多个ocs样元件。
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3
OCSBF-1, a maize ocs enhancer binding factor: isolation and expression during development.OCSBF-1，一种玉米ocs增强子结合因子：发育过程中的分离与表达
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Saturation mutagenesis of the octopine synthase enhancer: correlation of mutant phenotypes with binding of a nuclear protein factor.章鱼碱合酶增强子的饱和诱变：突变体表型与一种核蛋白因子结合的相关性
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Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide.生长素、水杨酸、茉莉酸甲酯和过氧化氢对as-1型元件和烟草谷胱甘肽S-转移酶基因的协同激活作用。
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10
Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences.不同类型的DNA结合蛋白之间的相互作用增强了与ocs元件启动子序列的结合。
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本文引用的文献

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Transcription in Isolated Wheat Nuclei: I. ISOLATION OF NUCLEI AND ELIMINATION OF ENDOGENOUS RIBONUCLEASE ACTIVITY.小麦核转录：I. 核的分离和内源性核糖核酸酶活性的消除。
Plant Physiol. 1980 Feb;65(2):305-8. doi: 10.1104/pp.65.2.305.
2
Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens.根癌土壤杆菌的Ti质粒导入植物的基因的孟德尔式传递。
Mol Gen Genet. 1981;183(2):209-13. doi: 10.1007/BF00270619.
3
A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.一种果蝇RNA聚合酶II转录因子含有启动子区域特异性DNA结合活性。
Cell. 1984 Feb;36(2):357-69. doi: 10.1016/0092-8674(84)90229-0.
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Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳研究乳糖阻遏物-操纵基因相互作用的平衡与动力学
Nucleic Acids Res. 1981 Dec 11;9(23):6505-25. doi: 10.1093/nar/9.23.6505.
5
Cell-specific activity of a GGTCA half-palindromic oestrogen-responsive element in the chicken ovalbumin gene promoter.鸡卵清蛋白基因启动子中GGTCA半回文雌激素反应元件的细胞特异性活性。
EMBO J. 1988 Dec 1;7(12):3771-8. doi: 10.1002/j.1460-2075.1988.tb03261.x.
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Cooperativity and hierarchical levels of functional organization in the SV40 enhancer.SV40增强子中的协同作用与功能组织的层次水平
Cell. 1988 Sep 23;54(7):943-53. doi: 10.1016/0092-8674(88)90109-2.
7
The SV40 enhancer contains two distinct levels of organization.猴病毒40增强子包含两个不同层次的组织。
Nature. 1988 May 5;333(6168):40-5. doi: 10.1038/333040a0.
8
Saturation mutagenesis of the octopine synthase enhancer: correlation of mutant phenotypes with binding of a nuclear protein factor.章鱼碱合酶增强子的饱和诱变：突变体表型与一种核蛋白因子结合的相关性
Proc Natl Acad Sci U S A. 1989 May;86(10):3733-7. doi: 10.1073/pnas.86.10.3733.
9
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.

一种DNA结合蛋白因子可识别章鱼碱合酶增强子元件内的两个结合结构域。

A DNA-binding protein factor recognizes two binding domains within the octopine synthase enhancer element.

作者信息

Tokuhisa J G, Singh K, Dennis E S, Peacock W J

机构信息

Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, Canberra, Australia.

出版信息

Plant Cell. 1990 Mar;2(3):215-24. doi: 10.1105/tpc.2.3.215.

DOI:10.1105/tpc.2.3.215
PMID:2152113
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC159878/
Abstract

A protein that binds to the enhancing element of the octopine synthase gene has been identified in nuclear extracts from maize cell suspension cultures. Two protein-DNA complexes are distinguishable by electrophoretic mobility in gel retardation assays. Footprint analyses of these low and high molecular weight complexes show, respectively, half and complete protection of the ocs-element DNA from cleavage by methidiumpropyl-EDTA.FE(II). Two lines of evidence indicate that the element has two recognition sites, each of which can bind identical protein units. Elements that are mutated in one or the other half and form only the low molecular weight complex interfere with the formation of both the low and high molecular weight complexes by the wild-type element. Protein isolated from a complex with only one binding site occupied can bind to the wild-type ocs-element and generate complexes with protein occupying one or both binding sites. Occupation of both sites of the ocs-element is a prerequisite for transcriptional enhancement.

摘要

在玉米细胞悬浮培养物的核提取物中已鉴定出一种与章鱼碱合酶基因增强元件结合的蛋白质。在凝胶阻滞试验中，通过电泳迁移率可区分出两种蛋白质-DNA复合物。对这些低分子量和高分子量复合物的足迹分析分别显示，ocs元件DNA的一半和全部受到甲哌鎓丙基-EDTA.Fe(II)切割的保护。有两条证据表明该元件有两个识别位点，每个识别位点都能结合相同的蛋白质单元。在其中一半发生突变且仅形成低分子量复合物的元件会干扰野生型元件形成低分子量和高分子量复合物。从仅占据一个结合位点的复合物中分离出的蛋白质可以与野生型ocs元件结合，并产生蛋白质占据一个或两个结合位点的复合物。ocs元件两个位点都被占据是转录增强的先决条件。