Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
J Pharmacol Sci. 2011;116(1):128-31. doi: 10.1254/jphs.11011sc. Epub 2011 Apr 27.
To elucidate the role of Na(+)/Ca(2+) exchanger (NCX) in neurite outgrowth, we investigated the effects of NCX inhibitors on neurite outgrowth in PC12 cells. KB-R7943 and 3',4'-dichlorobenzamil, NCX inhibitors, inhibited the neurite outgrowth caused by nerve growth factor (NGF). NCX inhibitors inhibited the neurite outgrowth caused by dibutylyl cAMP, which rapidly reorganizes the cytoskeleton. KB-R7943 inhibited the neurite outgrowth caused by Y-27632, an inhibitor of Rho kinase (ROCK) that regulates actin. However, NCX inhibitors did not inhibit NGF-induced phosphorylation of extracellular signal-regulated kinase. These results suggest that NCX inhibitor affects downstream of the Rho-ROCK signal transduction pathways in neurite outgrowth.
为了阐明钠钙交换器（NCX）在轴突生长中的作用，我们研究了 NCX 抑制剂对 PC12 细胞轴突生长的影响。NCX 抑制剂 KB-R7943 和 3',4'-二氯苯甲脒抑制神经生长因子（NGF）引起的轴突生长。NCX 抑制剂抑制了二丁酰环腺苷酸引起的轴突生长，二丁酰环腺苷酸可快速重组细胞骨架。KB-R7943 抑制了 Rho 激酶（ROCK）抑制剂 Y-27632 引起的轴突生长，ROCK 调节肌动蛋白。然而，NCX 抑制剂不抑制 NGF 诱导的细胞外信号调节激酶磷酸化。这些结果表明，NCX 抑制剂影响 Rho-ROCK 信号转导通路下游的轴突生长。
