胃癌中 miR-622 的下调通过靶向 ING1 基因促进细胞侵袭和肿瘤转移。
Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene.
机构信息
Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Weiqi Road, Jinan 250021, Shandong Province, China.
出版信息
World J Gastroenterol. 2011 Apr 14;17(14):1895-902. doi: 10.3748/wjg.v17.i14.1895.
AIM
To evaluate the biological and clinical characteristics of miR-622 in gastric cancer.
METHODS
We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family, member 1 (ING1) expression.
RESULTS
Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentiation and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion, tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. ING1 is a direct target of miR-622.
CONCLUSION
These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona fide treatment of gastric cancer.
目的
评估 miR-622 在胃癌中的生物学和临床特征。
方法
通过定量实时聚合酶链反应分析了 57 对配对的胃癌肿瘤和相邻非肿瘤组织中 miR-622 的表达。通过 miR-622 前体和抑制剂在胃癌细胞系中进行体外功能分析。利用稳定的 miR-622 表达质粒在裸鼠中分析 miR-622 在肿瘤发生和肿瘤转移中的作用。利用荧光素酶报告基因测定分析 miR-622 对生长抑制因子家族成员 1（ING1）表达的影响。
结果
miR-622 在胃癌中表达下调。miR-622 参与了人类胃癌的分化和淋巴转移。miR-622 的异位表达促进了胃癌细胞在体外和体内的侵袭、肿瘤发生和转移。ING1 是 miR-622 的直接靶标。
结论
这些发现有助于阐明胃癌转移涉及的分子机制，并表明 miR-622 的调节可能是治疗胃癌的一种有效方法。
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