Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Weiqi Road, Jinan 250021, Shandong Province, China.
World J Gastroenterol. 2011 Apr 14;17(14):1895-902. doi: 10.3748/wjg.v17.i14.1895.
To evaluate the biological and clinical characteristics of miR-622 in gastric cancer.
We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family, member 1 (ING1) expression.
Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentiation and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion, tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. ING1 is a direct target of miR-622.
These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona fide treatment of gastric cancer.
评估 miR-622 在胃癌中的生物学和临床特征。
通过定量实时聚合酶链反应分析了 57 对配对的胃癌肿瘤和相邻非肿瘤组织中 miR-622 的表达。通过 miR-622 前体和抑制剂在胃癌细胞系中进行体外功能分析。利用稳定的 miR-622 表达质粒在裸鼠中分析 miR-622 在肿瘤发生和肿瘤转移中的作用。利用荧光素酶报告基因测定分析 miR-622 对生长抑制因子家族成员 1（ING1）表达的影响。
miR-622 在胃癌中表达下调。miR-622 参与了人类胃癌的分化和淋巴转移。miR-622 的异位表达促进了胃癌细胞在体外和体内的侵袭、肿瘤发生和转移。ING1 是 miR-622 的直接靶标。
这些发现有助于阐明胃癌转移涉及的分子机制，并表明 miR-622 的调节可能是治疗胃癌的一种有效方法。
