Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, Shandong Province, China.
World J Gastroenterol. 2011 Apr 21;17(15):2037-43. doi: 10.3748/wjg.v17.i15.2037.
To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association between DEC1 expression and histopathological variables and the role of DEC1 in hepatocarcinogenesis.
The expression of DEC1 was detected immunohistochemically in 176 paraffin-embedded sections from 63 patients with HCC and 50 subjects with normal liver tissues.
DEC1 protein was persistently expressed in the cytoplasm of hepatocytes in normal liver and HCC tissues. Compared with adjacent non-tumor liver tissues, HCC tissues showed high nuclear expression of DEC1 protein. However, high DEC1 nuclear expression was more frequently detected in well-differentiated (83.3%) than in moderately (27.3%) and poorly differentiated HCC (16.7%). Low DEC1 expression was associated with poor histological differentiation and malignancy progression. A correlation was found between the nuclear expression of DEC1 protein and histological differentiation (r = 0.376, P = 0.024).
DEC1 is expressed in the cytoplasm of hepatocytes and because nuclear DEC1 expression is decreased with decreasing differentiation status of HCC, nuclear DEC1 might be a marker of HCC differentiation.
研究人分化胚胎软骨细胞 1（DEC1）在肝细胞癌（HCC）及相应癌旁非肿瘤和正常肝组织中的表达模式，探讨 DEC1 表达与组织病理学变量的关系及其在肝癌发生中的作用。
采用免疫组织化学方法检测 63 例 HCC 患者和 50 例正常肝组织石蜡包埋标本中 DEC1 的表达。
DEC1 蛋白在正常肝组织和 HCC 组织的肝细胞胞质中持续表达。与癌旁非肿瘤肝组织相比，HCC 组织中 DEC1 蛋白的核表达较高。然而，高 DEC1 核表达更常见于高分化（83.3%）HCC，而非中分化（27.3%）和低分化 HCC（16.7%）。低 DEC1 表达与组织学分化差和恶性进展有关。DEC1 蛋白的核表达与组织学分化呈正相关（r = 0.376，P = 0.024）。
DEC1 表达于肝细胞的胞质中，且由于 HCC 分化程度越低 DEC1 的核表达越低，因此核 DEC1 可能是 HCC 分化的标志物。
