Urtreger A, Ladeda V, Puricelli L, Rivelli A, Vidal M, Delustig E, Joffe E
Int J Oncol. 1997 Sep;11(3):489-96. doi: 10.3892/ijo.11.3.489.
Two cell lines obtained by sequential subcultures from primary cultures of the murine mammary adenocarcinomas M3 and MM3 were characterized. During LM3 cell line evolution an increase in the anchorage-independent growth ability, a displacement towards a triploid level in the chromosomic distribution and a loss of fibronectin (FN) expression were observed. LMM3 cell line showed a tetraploid chromosomic number and was unable to produce FN, behaving in a similar way as its parental tumor MM3. The local invasive ability as well as the spontaneous metastatic capacity of LM3 cells inoculated subcutaneously (s.c.) increased with the passage number and were associated with the progressive loss of FN expression as well as to the appearence of membrane bound uPA. LMM3 cells, which did not express FN, maintained the high spontaneous metastasic capacity already shown by the parental tumor MM3 but acquired a higher local invasive phenotype.
对从小鼠乳腺腺癌M3和MM3原代培养物中通过连续传代获得的两种细胞系进行了表征。在LM3细胞系的演变过程中，观察到其非贴壁依赖性生长能力增强、染色体分布向三倍体水平偏移以及纤连蛋白（FN）表达缺失。LMM3细胞系显示四倍体染色体数，且无法产生FN，其行为与其亲本肿瘤MM3相似。皮下接种的LM3细胞的局部侵袭能力以及自发转移能力随传代次数增加，并且与FN表达的逐渐丧失以及膜结合型尿激酶型纤溶酶原激活剂（uPA）的出现有关。不表达FN的LMM3细胞维持了亲本肿瘤MM3已显示出的高自发转移能力，但获得了更高的局部侵袭表型。
