4T1细胞的传代次数影响4T1原位小鼠肿瘤的发展和转移进程。
Passage Number of 4T1 Cells Influences the Development of Tumour and the Progression of Metastasis in 4T1 Orthotopic Mice.
作者信息
Rajaratinam Harishini, Rasudin Nur Syahmina, Safuan Sabreena, Abdullah Nurul Asma, Mokhtar Noor Fatmawati, Mohd Fuad Wan Ezumi
机构信息
School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Kelantan, Malaysia.
出版信息
Malays J Med Sci. 2022 Jun;29(3):30-42. doi: 10.21315/mjms2022.29.3.4. Epub 2022 Jun 28.
BACKGROUND
The study was aimed to elucidate the influence of passage number of 4T1 cells for the development of the ideal tumour model.
METHODS
A total of 24 female BALB/c mice was divided equally into three groups: i) control (phosphate buffered saline [PBS] only); ii) group A (subjected to 4T1 cells of passage number 9) and iii) group B (subjected to 4T1 cells of passage number 10). The injections were introduced at the 3rd mammary pad of the mice. The net volume of the tumours was examined. Histopathological analysis was conducted to compare the extent of metastasis in the different groups of mice.
RESULTS
Group B had a higher net volume of 4T1 tumour as compared to group A ( = 0.042). The coefficient of variation in the net volume of 4T1 tumour for group A was higher (135.3%) as compared to group B (40.79%). Group A only exhibited metastasis on the lungs, liver and spleen whereas group B showed metastasis to the heart, spleen, lungs and liver.
CONCLUSION
The use of 4T1 cells from passage number 10 is more ideal for the development of 4T1 tumour.
背景
本研究旨在阐明4T1细胞传代次数对理想肿瘤模型建立的影响。
方法
将24只雌性BALB/c小鼠平均分为三组：i）对照组（仅注射磷酸盐缓冲液[PBS]）；ii）A组（注射传代9次的4T1细胞）和iii）B组（注射传代10次的4T1细胞）。在小鼠的第三乳腺垫处进行注射。检测肿瘤的净体积。进行组织病理学分析以比较不同组小鼠的转移程度。
结果
与A组相比，B组的4T1肿瘤净体积更大（P = 0.042）。A组4T1肿瘤净体积的变异系数（135.3%）高于B组（40.79%）。A组仅在肺、肝和脾出现转移，而B组则出现了向心脏、脾、肺和肝的转移。
结论
使用传代10次的4T1细胞更适合建立4T1肿瘤模型。
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