Department of Biochemistry & Molecular Biology, University of Oklahoma Health Science Center, Oklahoma City, OK 73104, USA.
Lung Cancer. 2011 Nov;74(2):206-11. doi: 10.1016/j.lungcan.2011.03.014. Epub 2011 May 6.
The goal of this study was to evaluate the usefulness of electrospray ionization-mass spectrometry (ESI-MS) technology to distinguish sera of early-stage lung cancer patients from control individuals. ESI-MS m/z (mass divided by charge) data were generated from sera of 43 non-small cell lung cancer patients (pathological stages I and II) and 21 control individuals. Identifications of m/z peak area significances between cancer and control ESI-MS sera spectra were performed using t-tests. A "leave one out" cross validation procedure, which mimics blinded sera analysis and corrects for "over-fitting" of data, yielded discriminatory cancer versus control distribution p value and ROC curve area value of <0.001 and 0.87, respectively. Analysis without the "leave one out" cross validation procedure yielded a ROC curve area of 0.99 for discrimination of sera from lung cancer patients versus control individuals. Predictive value measurements revealed overall test efficiency and sensitivity for distinguishing sera from lung cancer patients from controls (using "leave one out" cross validation) of 80% and 84%, respectively. ESI-MS serum analysis between control individuals and lung cancer patients who smoked or did not smoke had p values in ranges indicating that smoking effects are not pronounced in our analysis. These studies indicate that ESI-MS analyses of sera from early stage non-small cell lung cancer patients were helpful in distinguishing these patients from control individuals.
本研究旨在评估电喷雾电离-质谱（ESI-MS）技术在区分早期肺癌患者血清与对照个体方面的有用性。ESI-MS 的 m/z（质量与电荷之比）数据来自 43 例非小细胞肺癌患者（病理分期 I 期和 II 期）和 21 例对照个体的血清。使用 t 检验对癌症和对照 ESI-MS 血清光谱中 m/z 峰面积显著性进行鉴定。“留一法”交叉验证程序模拟了盲血清分析，并纠正了数据的“过度拟合”，得出区分癌症与对照的分布 p 值和 ROC 曲线面积值分别为 <0.001 和 0.87。没有“留一法”交叉验证程序的分析得出的 ROC 曲线面积为 0.99，用于区分肺癌患者与对照个体的血清。预测值测量显示，使用“留一法”交叉验证，区分肺癌患者与对照个体血清的总体测试效率和敏感性分别为 80%和 84%。对吸烟或不吸烟的对照个体和肺癌患者的 ESI-MS 血清分析的 p 值表明，在我们的分析中，吸烟的影响并不明显。这些研究表明，ESI-MS 分析早期非小细胞肺癌患者的血清有助于将这些患者与对照个体区分开来。
