Department of Clinical Gene Therapy, Osaka University, Yamadaoka, Osaka 565-0871, Japan.
Neuroscience. 2011 Jul 14;186:110-9. doi: 10.1016/j.neuroscience.2011.04.035. Epub 2011 Apr 20.
Microdialysis is a powerful in vivo technique for the continuous sampling of small molecules within the extracellular fluid space. However, efforts to collect larger molecules have met with little success. To identify biologically active larger molecules in free-moving animals would be of great benefit. For this purpose, we have developed a novel microdialysis method that allows consistent recovery of large molecules from the brain interstitial space in the awake, free-moving mouse. Using a new "vent" probe with a push-pull perfusion system, the present study successfully demonstrated in vivo sampling of pathophysiologically important macromolecules in free-moving mouse brain. This sampling system allowed monitoring of the dynamic changes in their concentrations. Overall, this novel microdialysis system would provide the opportunity to identify the expression patterns of pathophysiologically important proteins in a variety of physiological and pathological processes for a better understanding of various diseases.
微透析是一种强大的活体技术，可用于连续采集细胞外液空间中小分子的样本。然而，在收集较大分子方面的努力收效甚微。在活动的动物体内鉴定具有生物活性的较大分子将是非常有益的。为此，我们开发了一种新的微透析方法，可在清醒、自由活动的小鼠中从脑间质空间持续回收大分子。使用带有推挽灌注系统的新型“通风口”探头，本研究成功地在自由活动小鼠的脑中进行了体内采样，以获取生理病理重要的大分子。该采样系统允许监测其浓度的动态变化。总的来说，这种新型的微透析系统将为在各种生理和病理过程中鉴定生理病理重要蛋白的表达模式提供机会，从而更好地理解各种疾病。
