胃贲门腺癌及异型增生中 HLTF 基因 CpG 岛的异常甲基化。
Aberrant methylation of the CpG island of HLTF gene in gastric cardia adenocarcinoma and dysplasia.
机构信息
Department of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
出版信息
Clin Biochem. 2011 Jul;44(10-11):784-8. doi: 10.1016/j.clinbiochem.2011.04.006. Epub 2011 Apr 22.
OBJECTIVES
To investigate the promoter methylation of HLTF in the tissues and plasma of patients with gastric cardia adenocarcinoma (GCA) and dysplasia.
DESIGN AND METHODS
Nested MSP approach was used to detect HLTF methylation status.
RESULTS
The frequency of HLTF methylation in high grade dysplasia and GCA tumor tissues was significantly higher than that in chronic inflammation tissues and was significantly associated with upper gastrointestinal cancers (UGIC) family history and protein and mRNA expression of the gene. HLTF methylation was not found in the plasma of patients with chronic inflammation and low grade dysplasia, while 4.0% (1/25) of patients with high grade dysplasia and 20.8% of (20/96) GCA patients were detected with hypermethylation of HLTF in the plasma.
CONCLUSIONS
In all, HLTF methylation may exist in gastric cardia dysplasia stages and may play important role in the development of GCA especially in individuals with UGIC family history.
目的
检测胃贲门腺癌（GCA）及异型增生组织和血浆中 HLTF 的启动子甲基化。
设计和方法
采用巢式 MSP 法检测 HLTF 甲基化状态。
结果
高级别异型增生和 GCA 肿瘤组织中 HLTF 甲基化的频率明显高于慢性炎症组织，且与上消化道癌（UGIC）家族史及基因蛋白和 mRNA 表达显著相关。在慢性炎症和低级别异型增生患者的血浆中未发现 HLTF 甲基化，而在 4.0%（1/25）的高级别异型增生患者和 20.8%（20/96）的 GCA 患者的血浆中检测到 HLTF 过度甲基化。
结论
总之，HLTF 甲基化可能存在于胃贲门异型增生阶段，在 GCA 的发生发展中可能起重要作用，尤其是在具有 UGIC 家族史的个体中。
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