Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland.
J Chromatogr A. 2011 Jun 17;1218(24):3750-6. doi: 10.1016/j.chroma.2011.04.039. Epub 2011 Apr 21.
A HPLC-detector has been designed which employs light-emitting diodes in the deep-UV-range below 300 nm as wavelength specific radiation sources and special UV-photodiodes for measuring the signal. A monochromator is therefore not needed. The design features a beam splitter and a reference photodiode, precision mechanics for adjustment of the light beams and electronics for stabilization of the LED-current. The processing of the photodiode currents is carried out with a high performance log-ratio amplifier which allows direct absorbance measurements. The optical and electronic performance of the detector was characterised and high precision over several absorbance units was obtained. Testing of analytical separation methods in isocratic as well as gradient modes employing UV-detection at 255 and 280 nm showed a very similar performance to a commercial photodiode-array detector used in the fixed wavelength mode in terms of linearity, precision and detection limits. The chief advantages of the new device are small size, low power consumption, and low cost.
已设计出一种 HPLC 检测器，它在 300nm 以下的深紫外范围内使用发光二极管作为波长特定的辐射源，并用特殊的紫外光电二极管来测量信号。因此，不需要单色仪。该设计的特点是分光器和参考光电二极管、用于调整光束的精密机械以及用于稳定 LED 电流的电子设备。光电二极管电流的处理是用高性能的对数比放大器进行的，它允许直接进行吸光度测量。对检测器的光学和电子性能进行了表征，在几个吸光度单位上都获得了很高的精度。在等度和梯度模式下对分析分离方法的测试表明，在 255nm 和 280nm 处进行紫外检测时，其性能与商业光电二极管阵列检测器在固定波长模式下非常相似，在线性度、精度和检测限方面都是如此。该新设备的主要优点是体积小、功耗低、成本低。
